Protein Info for GFF1275 in Xanthobacter sp. DMC5

Annotation: 1-carboxybiuret hydrolase subunit AtzE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 55% identical to ATZE_PSESD: 1-carboxybiuret hydrolase subunit AtzE (atzE) from Pseudomonas sp. (strain ADP)

KEGG orthology group: K02433, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit A [EC: 6.3.5.6 6.3.5.7] (inferred from 84% identity to xau:Xaut_4656)

MetaCyc: 55% identical to 1-carboxybiuret hydrolase catalytic subunit (Pseudomonas sp. ADP)
Omega-amidase. [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]; 3.5.1.131 [EC: 3.5.1.131, 3.5.1.3]

Predicted SEED Role

"Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases"

MetaCyc Pathways

L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
ammonia assimilation cycle III (3/3 steps found)
L-glutamate biosynthesis I (2/2 steps found)
cyanuric acid degradation I (4/5 steps found)
L-glutamine degradation I (1/1 steps found)
L-citrulline biosynthesis (6/8 steps found)
L-glutamate and L-glutamine biosynthesis (5/7 steps found)
superpathway of L-citrulline metabolism (8/12 steps found)
L-methionine salvage cycle I (bacteria and plants) (6/12 steps found)
L-methionine salvage cycle II (plants) (5/11 steps found)
S-methyl-5-thio-α-D-ribose 1-phosphate degradation I (2/7 steps found)
L-methionine salvage cycle III (4/11 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7

Use Curated BLAST to search for 3.5.1.131 or 3.5.1.3 or 6.3.5.6 or 6.3.5.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>GFF1275 1-carboxybiuret hydrolase subunit AtzE (Xanthobacter sp. DMC5)
MNAPVPVPDATRFAGATAAEIAAAVASGTVTARAVIEATLARIAALDPAINAFTDLTAER
ARAHADRIDAARAAGEPLGPLAGVPFAVKNLFDIAGLPTRAGSKINRERAPSAQDAFLVE
KLEAAGAILVGGLNMGEYAYDFTGENVHDGASRNPHDLTRMSGGSSGGTGAAVAAAMVPI
ALGSDTNGSIRVPSSLCGTFGLKPTYGRLGRGGSFPFVTALDHLGPLARSVADLALSYDA
MQGEDARDPGQTPRPFEPVAGLIGEGIDGLRIAIAGGWFRAKAHPEAFAAVDAVAAALGA
RDMVDIPDAARARASAYIITAAEGASLHARRLAVRPEDFDPAVRDRLLAGLAIPASWVDA
AQRFRRHFRGEMLKLFETHDVLLAPATPSRAPKLGQKTFVLDGEEMLVRPNLGLFTQPIS
FIGLPVVAVPVRVDVGDAPDLPIGVQVIAPPWREDMALRVAAELERLGVAAAPAATL