Protein Info for GFF1271 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 575 (575 residues), 710.2 bits, E=1.2e-217
• PF00587: tRNA-synt_2b
  • amino acids 96 to 468 (373 residues), 105.9 bits, E=4.2e-34
• PF04073: tRNA_edit
  • amino acids 257 to 386 (130 residues), 95.8 bits, E=3e-31
• PF03129: HGTP_anticodon
  • amino acids 485 to 576 (92 residues), 71.3 bits, E=9.4e-24

Best Hits

Swiss-Prot: 86% identical to SYP_ACIAC: Proline--tRNA ligase (proS) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 86% identity to adn:Alide_0931)

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (6/25 steps found)
• phosphinothricin tripeptide biosynthesis (4/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.15

Protein Sequence (581 amino acids)

>GFF1271 Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKASQFLISTLKEAPADAEVVSHQLMTRAGMIKKLGAGIYSYMPMGLRVIHKVEAIVREE
MNRAGSVELTMPVIQPAEYWQETGRFDKMGAELLRIKDRHDRDFVVQPTSEEVVTDIARQ
DFRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDAYSFDRDESTAKLSYQVMAGA
YRRIFDRFGLRYRAVAADSGAIGGDLSEEFQVIAATGEDAIVYCPASDYAANMEKAEALA
PAGPRPAAANPMAKTPTPGKATCADVATLLGVPLATTVKSLVLATDELNEAGEIVKSQVW
LLLLRGDHDMNEVKVAKVPGLNAGFRFATGAEIDTHFGCKPGYLGPIGLKKPVNLVVDRE
VAVLADWICGANEPDFHMTGVNWGRDLPEPTLVADLRNVVEGDASPDGKGVLAIERGIEV
GHVFYLGTKYSKAMNATFLGEDGKPAHFEMGCYGIGITRLPAAAIEQNHDERGIIWPDAL
APFTVVVCPIGMDRSPEVKAAAEQLYGELLAAGVDVILDDRGERPGAMFADWELIGVPHR
VTIGDRGLKAGQVEYQHRRDAAATQVAVGDVVSLLKGKLGA