Protein Info for PS417_06430 in Pseudomonas simiae WCS417

Annotation: acyl-homoserine lactone acylase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF01804: Penicil_amidase" amino acids 52 to 799 (748 residues), 608.4 bits, see alignment E=1.4e-186

Best Hits

Swiss-Prot: 79% identical to QUIP_PSEPF: Acyl-homoserine lactone acylase QuiP (quiP) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01434, penicillin amidase [EC: 3.5.1.11] (inferred from 97% identity to pfs:PFLU1314)

Predicted SEED Role

"Penicillin acylase (EC 3.5.1.11)" (EC 3.5.1.11)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.11

Use Curated BLAST to search for 3.5.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U328 at UniProt or InterPro

Protein Sequence (813 amino acids)

>PS417_06430 acyl-homoserine lactone acylase subunit beta (Pseudomonas simiae WCS417)
MASPALSHFLPRFGVAAAVASALSLAGCQLQSTQDTLPPVAGVQPLKGLAQNVSVRRNAQ
GMPLIESNTFHDALFSLGYVHASDRITQMVTLRLLAQGRLAEMSGPEVLDVDRFMRAVNL
KKSAGELYNASSPRLKRFFEVYARGVNAYLFRYRDKLPPDLAQTGYKPEYWKPEDSALLF
CLLNFSESVNLQEEIASLVLAQKVGVDKLAWLTPSAPDEAIPLAEAEKLKGVNFSQITGL
AGLDAVGQQLSSLNSLAVTTSSNWAIGPQRSRSGKSLLAGDLAAQPQAPSPWSYVQIRAP
KYQAVGASIAGLPTLLSGFNGKVAWSMSTVKGDTQDLFLEKVKRQGSALYYENNGKWLPA
GVRNETFFVKGQRSIREVVYETRHGALLNSSQALTTGLGLALQTAELKDDKSLDAFFDLS
RAQNAGKASDATREIRAIALNMIFADASNIGWQVTGRFPNRREGEGLLPSPGWDTRFDWD
GYADAMLHPYDQDPAQGWIGTANQRTAPRGYGMQLSNSWDAPERSERLAQLANAGKHDSR
SMIAMQYDQTTTFAAKLKTMFQAPGMAQPLKQAIDALPAPEQTKAREALGRLMAFDGRLA
STSADAAIYELFLQESAKQIFLDELGPENSASWKAFVSNASLSYAAQADHLLGREDSPFW
DDVRTPQKEDKPAILARSLAAAISAGDSQLGADHKAWQWGKLHTTTWKNTNGQAIRGPLA
SGGDHNTLNPAPYSWGQDFTTTQVSALRMIVDFGQVEPMMGQDGIGQSGNPASPNYANGI
DPSLKAQYLSFPMQPQNFEKVYGKARLTLTPGK