Protein Info for PS417_06390 in Pseudomonas simiae WCS417

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 826 TIGR04121: DEXH box helicase, DNA ligase-associated" amino acids 8 to 818 (811 residues), 1142.2 bits, see alignment E=0 PF00270: DEAD" amino acids 22 to 200 (179 residues), 91.5 bits, see alignment E=1.3e-29 PF04851: ResIII" amino acids 25 to 170 (146 residues), 28.3 bits, see alignment E=3.8e-10 PF00271: Helicase_C" amino acids 247 to 355 (109 residues), 49 bits, see alignment E=1.6e-16 PF19306: Lhr_WH" amino acids 397 to 545 (149 residues), 187 bits, see alignment E=4.4e-59 PF08494: DEAD_assoc" amino acids 603 to 789 (187 residues), 175.6 bits, see alignment E=2.7e-55

Best Hits

KEGG orthology group: K03724, ATP-dependent helicase Lhr and Lhr-like helicase [EC: 3.6.4.-] (inferred from 93% identity to pfs:PFLU1306)

Predicted SEED Role

"FIG003033: Helicase domain protein"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UBF6 at UniProt or InterPro

Protein Sequence (826 amino acids)

>PS417_06390 DEAD/DEAH box helicase (Pseudomonas simiae WCS417)
MAKATDFAKRWFAAKGWKPFAFQKEVWAAVKSGQSGLLHASTGAGKTYALWFAALNRFAI
ARPPVTAKRKPPAEPLTVLWITPMRALAADTARALEAPLEALQIPWSVGLRTGDTSSSER
ARQTRRQPTVLVTTPESLTLMLARADSEVSLAHLRMVVVDEWHELIGNKRGVQLQLALAR
LRRWHPDLMVWGISATLGNQAHALEVLVPQGDGINVQGQTAKQLIIDTLLPPVTERFPWA
GHIGLKMLPQVVAEVDASSSCLVFTNTRAQSEIWFQALLDARPDWAGVIALHHGSLSRET
RDWVERALKDGQLKAVVCTSSLDLGVDFLPVERVLQIGSAKGVARLMQRAGRSGHAPGRP
SRVTLVPTHSLELVEAAAAQDAIAQRRIEARESPYKPLDVLVQHLVSMALGGGYKPDDLL
VEVRGAWAYHDLTDEDWAWALGFVRHGGLSLTAYPDYRRVEPDEHGIWRVPDARLARRHR
MSVGTIVSDASIHLKFWSKGGGGKNLGSVEEGFIARLKPGDGFLFAGRLLELVRVENMTA
YVRRSNAKKAAVPRWNGGRMPLSNELAQAVVERFDAAAQGHFEGPEMQAVRPLLQTQVRW
SGLPTREHLLAEALKSREGWHLFLYPFAGRQVHLGLASLLAWRVSQGQAVTFSIAVNDYG
LELLSATEVNWPALLNEALLSPEHLLEDVVASLNAGELALRRFREIARIAGLVFAGYPGA
PKSTRQVQASSGLFFEVFKQYDPQNLLLTQAGEEVLRDELDIRRLEQTLRHLCALKLELH
RIQRPTPLAFPLLVERMRESMSSEKLSERIARMVKDLEKVADNGKR