Protein Info for GFF1255 in Sphingobium sp. HT1-2

Annotation: Chain length determinant exopolysaccharide biosynthesis protein EpsF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 35 to 35 (1 residues), see Phobius details amino acids 387 to 409 (23 residues), see Phobius details PF13807: GNVR" amino acids 331 to 408 (78 residues), 40.1 bits, see alignment E=1.5e-14

Best Hits

KEGG orthology group: None (inferred from 84% identity to sch:Sphch_0970)

Predicted SEED Role

"lipopolysaccharide biosynthesis"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (451 amino acids)

>GFF1255 Chain length determinant exopolysaccharide biosynthesis protein EpsF (Sphingobium sp. HT1-2)
MTMNPLDLAAAVKARWRVAAMIGGALFLLIAVAAFMQSRQYMATTSLMLDLSQTDPTDTT
QNQGRVEVDSILGTQTDIIRSAKVINAVAKEAGFIDALPADMPADARLQQAAARVRAGLL
VTTGRQSNVLQIQYLDPDPQVAARVANLAAQIYMREQVELRASPARTSAKWFNEQTADVR
RRYEDAQKRLSDFQRAHDIIGINRMDLEAEKLKNMSYQLTQAQAEAAAARGKAGAGSVSD
IEGSLIVQNLQEQVAAQSAKVQELGKTLGPNHPTMAAASAQLSELQAKLGAARASQAGAV
SANSVAASRREGDLKSNMASQEDRMIRMSDVQDQLMVMQRDVDAARQTYDTVRQRFNEAA
LKSQISQPNASLLDEASVPLLPAKPNLLLWLVGGIALGLVGGIAAVVLIEIAQPRVRSAA
GVARATEVEVITELLPAPARGGWLPKRQEAA