Protein Info for GFF1250 in Variovorax sp. SCN45

Annotation: Alkanesulfonate ABC transporter substrate-binding protein SsuA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details TIGR01728: ABC transporter, substrate-binding protein, aliphatic sulfonates family" amino acids 36 to 309 (274 residues), 214 bits, see alignment E=1.4e-67 PF13379: NMT1_2" amino acids 58 to 197 (140 residues), 35.8 bits, see alignment E=1.2e-12 PF04069: OpuAC" amino acids 60 to 230 (171 residues), 25.5 bits, see alignment E=1.5e-09 PF09084: NMT1" amino acids 67 to 231 (165 residues), 51.5 bits, see alignment E=2e-17

Best Hits

KEGG orthology group: K02051, sulfonate/nitrate/taurine transport system substrate-binding protein (inferred from 88% identity to vpe:Varpa_2670)

Predicted SEED Role

"Alkanesulfonates-binding protein" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization or Putative sulfate assimilation cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (318 amino acids)

>GFF1250 Alkanesulfonate ABC transporter substrate-binding protein SsuA (Variovorax sp. SCN45)
MTLISAPRRRLIQGVAAAVALPSFAALAQAPARQFRIGNQKGYLTLLKGRGTLEKRLAPL
GVSVKWTEFTAGPVQLEALNVGSIDFGDVGEAPPIFAQAAGAPLAYVGATVVRPQSEAVL
VPKASAIKTVAELKGKKIALNKGSNVHYFIVKLFEKHGLSYADLNLVYLPPADARAAFEK
GSVDAWVIWDPFLAAAEKSLDARILADATGVVGNRAYYFSSLDYVAKNADVLAIAIEELN
KIDVWGAANKAELAGELAALWGLPKPVAELSVNRSAYGTTPITKAILAEQQKIADTFFDL
KLIPKKINVLEAAGAGIA