Protein Info for PS417_06335 in Pseudomonas simiae WCS417

Annotation: S-formylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 5 to 279 (275 residues), 423.7 bits, E=1.7e-131
• PF00756: Esterase
  • amino acids 23 to 273 (251 residues), 232.1 bits, E=4.7e-73

Best Hits

Swiss-Prot: 61% identical to SFGH2_ECO57: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli O157:H7

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 98% identity to pfs:PFLU1296)

MetaCyc: 60% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Sequence Analysis Tools

See A0A1N7TYT9 at UniProt or InterPro

Protein Sequence (281 amino acids)

>PS417_06335 S-formylglutathione hydrolase (Pseudomonas simiae WCS417)
MSLENLSCQKSFGGWHKRYKHHSDVLGCDMTFAVYLPPQAEQGSKLPVLYWLSGLTCTDE
NFMQKAGAQRMAAELGLIIVAPDTSPRGPGVPGDPDNAWDFGLGAGFYLNATQEPWAKHY
RMHDYVVQELPALVEAHFPASDKRGISGHSMGGHGALVCALRNPGRYLSVSAFSPINNPM
DCPWGQKAFSRYLGEERSKWREWDACVLISEASEKLPLLVDQGDRDDFLAVQLKPEALQQ
AAKAANHPLELRLQPGYDHSYFFIASFIEDHLRHHGRALLG