Protein Info for GFF1245 in Xanthobacter sp. DMC5

Annotation: NADH-quinone oxidoreductase chain 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01959: NADH oxidoreductase (quinone), F subunit
  • amino acids 8 to 416 (409 residues), 623.8 bits, E=4.6e-192
• PF01512: Complex1_51K
  • amino acids 48 to 216 (169 residues), 164.5 bits, E=2.5e-52
• PF22461: SLBB_2
  • amino acids 240 to 303 (64 residues), 49.8 bits, E=4.6e-17
• PF10589: NADH_4Fe-4S
  • amino acids 332 to 414 (83 residues), 108.2 bits, E=2.4e-35

Best Hits

Swiss-Prot: 76% identical to NUOF1_RHIME: NADH-quinone oxidoreductase subunit F 1 (nuoF1) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 94% identity to xau:Xaut_4628)

MetaCyc: 71% identical to NADH-ubiquinone oxidoreductase 51 kDa subunit (Arabidopsis thaliana col)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Protein Sequence (434 amino acids)

>GFF1245 NADH-quinone oxidoreductase chain 1 (Xanthobacter sp. DMC5)
MLADKDRIFTNLYGFHDFGLQGALKRGNWDGTKAILEKGRDWIINEMKASGLRGRGGAGF
STGMKWSFMPKQSDGRPHYLVVNADESEPGTCKDREIMRNDPHTLIEGCLIASFAMGAHA
AYIYIRGEYIFERERLQAAVDQAYEARLIGKDNVHDYPFDLYVHHGAGAYICGEETALLE
SLEGKKGMPRLKPPFPANMGLYGCPTTVNNVESIAVAPTILRRGAAWFSSIGRPNNVGTK
LFSLAGHVNTPCVVEEAMSIPFKELVEKHGGGIRGGWDNLLGIIPGGASCPVIPGEQCED
LIMDFDGTRAVKSSFGTAGVLVMDKSTDIIKAIARISAFFKHESCGQCTPCREGTGWMWR
VVNRMAEGRAQKREIDLLLEVTTQIEGHTICALGDAAAWPVQGLIRHYRHVIEARIDKYA
ANPHPEPVVPVAAE