Protein Info for GFF1244 in Xanthobacter sp. DMC5

Annotation: NADH-quinone oxidoreductase chain 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 PF13510: Fer2_4" amino acids 3 to 69 (67 residues), 50.4 bits, see alignment E=6.7e-17 TIGR01973: NADH dehydrogenase (quinone), G subunit" amino acids 4 to 600 (597 residues), 737.2 bits, see alignment E=9.2e-226 PF00111: Fer2" amino acids 6 to 57 (52 residues), 32.7 bits, see alignment 2e-11 PF10588: NADH-G_4Fe-4S_3" amino acids 92 to 131 (40 residues), 65.9 bits, see alignment 6.4e-22 PF22117: Fer4_Nqo3" amino acids 145 to 211 (67 residues), 112.5 bits, see alignment E=3.1e-36 PF22151: Fer4_NDSU1" amino acids 226 to 274 (49 residues), 96.5 bits, see alignment 1.7e-31 PF00384: Molybdopterin" amino acids 280 to 603 (324 residues), 344.2 bits, see alignment E=2.9e-106 PF09326: NADH_dhqG_C" amino acids 636 to 679 (44 residues), 55.8 bits, see alignment 2e-18

Best Hits

KEGG orthology group: K00336, NADH dehydrogenase I subunit G [EC: 1.6.5.3] (inferred from 91% identity to xau:Xaut_4627)

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>GFF1244 NADH-quinone oxidoreductase chain 3 (Xanthobacter sp. DMC5)
MAKIVVDGTEVDVPAEYTLLQACEAAGVEIPRFCFHERLSIAGNCRMCLVEVKGGPPKPT
ASCAMAVKDLRPGPNGEPPVVLTKSPMVKKAREGVMEFLLINHPLDCPICDQGGECDLQD
QAMAYGADTSRFAENKRAVEDKYIGPLVKTSMNRCIQCTRCVRFSTEVAGVPDLGAIGRG
EDMEITTYLEHAMRSEMQGNVADLCPVGALTHKPYQFHARPWELVKTESIDVMDAVGSNI
RIDTRGREVMRFLPRNNDAVNEEWISDKSRYVWDGLKVQRLDRPYVRKDGRLVPATWSEA
FAAIAAKVKGTAPARIGALIGDLASVEEVFALKTLMGKLGSANIDARQDGAKLDPALGRA
SYVFNPTIAGIEQADAILLVGSDPRHEASVINSRIRKRWRQGGLKIGLIGTKVDLTYTYD
YLGAGPDSLADLAGSSAFGDILRKAERPLVIVGQGALARPDGAAVLSLAARVAVAVGAVK
DGWNGFGVLHTAAARVGALDVGAVPGAGGLDVAAMTAPGALDVAFLMGADEVEVAPGAFV
VYLGTHGDRGAHRADVILPGAAYPEKSGIYVNTEGRVQFANRAAFPPGEAREDWAILRAL
SEVLGQTLPFDNMQALRAQLVSAYPHLGHIDEAVEADAGAVSALAGVGGSVDKTAFAPVI
EDFFLTNPIARASAVMAECSALRLAAAAEAAE