Protein Info for GFF1231 in Xanthobacter sp. DMC5

Annotation: Iron-binding protein IscA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 132 PF01521: Fe-S_biosyn" amino acids 20 to 120 (101 residues), 69.6 bits, see alignment E=1.3e-23 TIGR00049: iron-sulfur cluster assembly accessory protein" amino acids 20 to 122 (103 residues), 112.6 bits, see alignment E=4.9e-37

Best Hits

Swiss-Prot: 49% identical to ISAM3_ARATH: Iron-sulfur assembly protein IscA-like 3, mitochondrial (At2g36260) from Arabidopsis thaliana

KEGG orthology group: K13628, iron-sulfur cluster assembly protein (inferred from 80% identity to xau:Xaut_4614)

Predicted SEED Role

"Iron binding protein SufA for iron-sulfur cluster assembly"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (132 amino acids)

>GFF1231 Iron-binding protein IscA (Xanthobacter sp. DMC5)
MSVAETPPARPARRSLPPVIRITDIAAERLREILAESEPGTAVRVGIENGGCAGMSYKLD
FTTDVAPLDAVVEDKGVKVVVDSKAVLFLIGTEMDFKTDKVSSQFVFNNPNQTSSCGCGE
SVAITPVAVDAS