Protein Info for GFF1225 in Sphingobium sp. HT1-2

Annotation: Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00358: PTS_EIIA_1" amino acids 9 to 129 (121 residues), 135.5 bits, see alignment E=2.1e-43 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 11 to 128 (118 residues), 121.8 bits, see alignment E=2.5e-39 TIGR01003: phosphocarrier, HPr family" amino acids 171 to 250 (80 residues), 67.4 bits, see alignment E=1.3e-22 PF00381: PTS-HPr" amino acids 171 to 250 (80 residues), 75.2 bits, see alignment E=8.8e-25 PF05524: PEP-utilisers_N" amino acids 275 to 383 (109 residues), 57 bits, see alignment E=5.5e-19 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 278 to 816 (539 residues), 422.3 bits, see alignment E=4.1e-130 PF00391: PEP-utilizers" amino acids 410 to 480 (71 residues), 62.1 bits, see alignment E=7.3e-21 PF02896: PEP-utilizers_C" amino acids 512 to 793 (282 residues), 328.1 bits, see alignment E=1.2e-101

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 50% identity to ccs:CCNA_00457)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (822 amino acids)

>GFF1225 Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) (Sphingobium sp. HT1-2)
MSGMMASVTLRAPLAGWLAPIKSVPDPVFAERMMGEGFAIDPIEGEVRAPADATVLTVAP
TGHSVSLRLANGAELLIHVGLETVTLGGKGFAPQVKPGDAVAAGDLLIGFDLDAVAEGAK
ALITPVVLAGEGYALSLEPLDRLVGWQDGVARITALAPVAAKGDSEGDSHERVVRVDAPH
GIHARPAARIAALLRTFVAPVAIVRDGKSVNARSTVALLGLGVRSGDEIIIRGEGSDARA
AVEALVALIEAGLGEEAKADHPAPAPVVPQHGPVTAAPGLAIGQVVQLRVADVDVPRDGQ
GGTAEHAALARAMAAVDAELSAGHGLAAEIAAAHRALLADPELAEAAGHQIDAGRSAAFA
WRHATAQAAEAIRATGDPLLMERVADLVDIERQLIAALLGNDASAVPTLPPQSILIAEDL
LPSQFLALDRDRLAGICTAAGGPTSHVAILAASAGIPMLVAAGRDVLGIAEGRTVILDAD
GARIDADPGVNTLSEVSARIAAAREQRSRDRAQAHADCRMADGTRIEIFANLGSQADAAA
AVAAGAEGCGLLRTEFLFLERAEAPDEAEQREIYSGIATTLGDRPLIVRTLDIGGDKPVP
YLPMAVEENPALGLRGVRLSLARPDLMQVQLRAILRAVPADQCRVMLPMIADLSDYRAVK
AMLDAEKAALGIDAPVPLGVMIETPAAAMLADMLAAEADFLSVGTNDLTQYTLAVDRGNA
AVSHRIDALHPAVLRLIREVGHGAQRHGRWAGVCGGLASDPLAAPILIGLGITELSATPA
AIARLKAVVRTLDMDRCIDLAERACAAESAAAVREMAQGVLA