Protein Info for PS417_06165 in Pseudomonas simiae WCS417

Annotation: thiamine biosynthesis protein ThiF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 269 PF00899: ThiF" amino acids 14 to 254 (241 residues), 145.4 bits, see alignment E=8e-47

Best Hits

Swiss-Prot: 56% identical to TCDA_ECOLI: tRNA threonylcarbamoyladenosine dehydratase (tcdA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 99% identity to pfs:PFLU1261)

MetaCyc: 56% identical to tRNA threonylcarbamoyladenosine dehydratase (Escherichia coli K-12 substr. MG1655)
RXN0-7115

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UDX3 at UniProt or InterPro

Protein Sequence (269 amino acids)

>PS417_06165 thiamine biosynthesis protein ThiF (Pseudomonas simiae WCS417)
MSTEDPRFAGVARLYGIEGLERLKAAHVAIVGVGGVGSWAAEAIARCGVGEISLFDLDDV
CVSNSNRQLHALDSTVGKPKVEVMAERLRGINPDCTVHAVADFVTRDTMAEYITPTIDCV
IDCIDAVNAKAALIAWCKRRKIQIITTGGAGGQIDPTLIQVCDLNRTFNDPLASKVRSTL
RRDYGFSRTVTRHYSVPCVFSTEQLRYPKPDGSICLQKSFVGDGVKLDCAGGFGAVMMVT
ATFGMVAATKAVDKIVAGVRRPSERVKPT