Protein Info for GFF1212 in Sphingobium sp. HT1-2

Annotation: Histidine ammonia-lyase (EC 4.3.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 85% identical to HUTH_CAUVC: Histidine ammonia-lyase (hutH) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 85% identity to cse:Cseg_3333)

MetaCyc: 54% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

L-histidine degradation II (5/5 steps found)
L-histidine degradation VI (7/8 steps found)
L-histidine degradation I (4/4 steps found)
L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (509 amino acids)

>GFF1212 Histidine ammonia-lyase (EC 4.3.1.3) (Sphingobium sp. HT1-2)
MNAIILVPGEASLADWRAIYRGATARLDGASAPRIAQSAAAVARILAKGEPVYGINTGFG
KLASVRIGDEDLATLQRNIVLSHAAGTGAPSPVPVIRLMMTLKLASLGQGASGVKPQTVA
LLEAMLVQGLTPVVPAQGSVGASGDLAPLSHMAAAMIGVGDIMLGEERLPAAEALARAGL
APLELGPKEGLALLNGTQFSTANALAGLFEAETLFQSALVTGALSTEAAKGSDTPFDPRI
HALRRHAGQIETADALRGLMAGSAIRASHLKGDERVQDPYCLRCQPQVMGAALDLLRQAA
TTLAIEANGVSDNPLIFPESDEALSGGNFHAEPVAFAADMIALAICEIGSIAERRIAMLV
DPALSGLPAFLTPKPGLNSGFMIPQVTAAALVSENKQRAYPASVDSIPTSANQEDHVSMA
AHGARRLLDMAENATAVIGIELLAAVQGMDFHAPLTSSAALEAARACVRKSVPHLTDDRH
FHPDMEAANRIVRSGALVAAAGHALPVMG