Protein Info for GFF121 in Sphingobium sp. HT1-2

Annotation: Glutathione reductase (EC 1.8.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 TIGR01424: glutathione-disulfide reductase" amino acids 4 to 447 (444 residues), 618.6 bits, see alignment E=2.7e-190 PF07992: Pyr_redox_2" amino acids 6 to 319 (314 residues), 251.2 bits, see alignment E=3e-78 PF13738: Pyr_redox_3" amino acids 127 to 303 (177 residues), 57.9 bits, see alignment E=2e-19 PF00070: Pyr_redox" amino acids 170 to 248 (79 residues), 74.1 bits, see alignment E=2.2e-24 PF02852: Pyr_redox_dim" amino acids 339 to 445 (107 residues), 122 bits, see alignment E=2.8e-39

Best Hits

Swiss-Prot: 51% identical to GSHR_PSEAE: Glutathione reductase (gor) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00383, glutathione reductase (NADPH) [EC: 1.8.1.7] (inferred from 89% identity to sjp:SJA_C1-17090)

Predicted SEED Role

"Glutathione reductase (EC 1.8.1.7)" in subsystem Glutathione: Redox cycle (EC 1.8.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF121 Glutathione reductase (EC 1.8.1.7) (Sphingobium sp. HT1-2)
MSDFDFDLFVIGAGSGGVRASRVAAAHGAKVAVAEEFRVGGTCVIRGCVPKKLLIYGAHF
AEDLKDARRFGWNVPDCGFEWATLRDNVLADVDRLEGLYTNTLNSHKVELIAQRVTVTGP
HSVKLASGREVTAKVILVATGAWPLIPEVEGAEHGITSNEVFHLDECPKRIVIVGGGYIA
NEFAGIFHQFGSHVTMVNRGGTLLRGYDEQIRDRLLQISTMKGINFRFNAQMEKIEKKED
GTLCVHFKNGDPVAADIVLFATGRRPHTDGLGLENAGVEIDDKGAIKVDEYSRTNCESIY
AVGDVTDRLQLTPVAIREGHAFADTVFGNNPRTVDYSCVPSAVFSHPPLAGVGLTEAQAK
NKYGTVKVYTSDFRPMKNVLAGRDERALYKMVVDATTNKVVGLHMIGPDAPEILQAAAVA
VKAGLTKQDFDDTVALHPSMAEELVLLK