Protein Info for GFF1204 in Variovorax sp. SCN45

Annotation: Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01507: PAPS_reduct
  • amino acids 40 to 185 (146 residues), 95.7 bits, E=1.7e-31

Best Hits

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 93% identity to vpe:Varpa_2615)

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8)" in subsystem Cysteine Biosynthesis (EC 1.8.4.8)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.4.8

Use Curated BLAST to search for 1.8.4.8

Protein Sequence (206 amino acids)

>GFF1204 Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) (Variovorax sp. SCN45)
MSIDFTRINTELGRNAQGLVDWALGLGQNAIVTTNFRPFEAVILHMVTQVKRDVPVVWMD
NGYNTEATYRYADEVTKQLGLDLHIYLPRRSRAHREAVEGPTPALDDPRHAAFTEEVKLE
PFARALRETAPKVWFTALRATDTAVRAQMDPVSVNPDGLIKVAPLLHWSSKDLHEYCVKH
GLPNNFDYVDPTKGEDNRECGLHLAH