Protein Info for GFF119 in Sphingobium sp. HT1-2

Annotation: Signal peptidase I (EC 3.4.21.89)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 274 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details PF10502: Peptidase_S26" amino acids 9 to 245 (237 residues), 156.3 bits, see alignment E=3.6e-50 TIGR02227: signal peptidase I" amino acids 13 to 249 (237 residues), 147.4 bits, see alignment E=1.7e-47

Best Hits

KEGG orthology group: K03100, signal peptidase I [EC: 3.4.21.89] (inferred from 92% identity to sjp:SJA_C1-17120)

Predicted SEED Role

"Signal peptidase I (EC 3.4.21.89)" in subsystem Signal peptidase (EC 3.4.21.89)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.89

Use Curated BLAST to search for 3.4.21.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (274 amino acids)

>GFF119 Signal peptidase I (EC 3.4.21.89) (Sphingobium sp. HT1-2)
MSAKSETRDFIWFLAKLAVFVFVLRSFIVSPFNIPSESMQPRLLIGDYLLVAKWPYGYSR
YSLPFSVPLIPGRIFASTPQRGDVVVFKAPPSQKNDYIKRVIGLPGDMVSVRGGTVYLNG
QAIPKQKVADLVIPVTPNMEDAAQKEGSPSPCYRPEFEQAAPGGGKQCRYPQYRETLPGG
KSYNVLDLVPDGAADDRDTVLVPQGHLFMMGDNRDRSADSRFPAVEGGGIGLVPEENLVG
KALVSVFSTDGSANWLLPWTWFTAARWSRIGEGF