Protein Info for GFF1183 in Sphingobium sp. HT1-2

Annotation: Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 837 PF07992: Pyr_redox_2" amino acids 27 to 301 (275 residues), 196 bits, see alignment E=3.5e-61 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 28 to 800 (773 residues), 943.4 bits, see alignment E=6.4e-288 PF00070: Pyr_redox" amino acids 170 to 249 (80 residues), 61.9 bits, see alignment E=2.4e-20 PF18267: Rubredoxin_C" amino acids 338 to 405 (68 residues), 65.7 bits, see alignment E=1.1e-21 PF04324: Fer2_BFD" amino acids 443 to 490 (48 residues), 38 bits, see alignment 6e-13 amino acids 504 to 553 (50 residues), 42.8 bits, see alignment 1.8e-14 PF03460: NIR_SIR_ferr" amino acids 579 to 643 (65 residues), 57.4 bits, see alignment 3.4e-19 PF01077: NIR_SIR" amino acids 652 to 786 (135 residues), 112.6 bits, see alignment E=4.5e-36

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 88% identity to sjp:SJA_C1-28550)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (837 amino acids)

>GFF1183 Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4) (Sphingobium sp. HT1-2)
MDFQDGIDPVTQDEDNVLPPVGQAREHLVVIGNGMAGCRAVEELLARDAGRYRVTIFGAE
PYVNYNRIMLSPVLAGEKTFEQIVINDAAWYADNGIELIAGDPVKAIDRAAKTVSSQSGR
TIGYDKLLIATGSDPFIIPVPGKDLPGVISFRDMKDVDTMLAAAADGGSAVVIGGGLLGL
EAAHGLTLRGMKVTVIHLMDTLMERQLDEAAGWLLKTALEGRGQTILTGANTEAIYGDGK
VEGVRLKDGREIPASLVVMAVGIRPATALAREAGLAVNRGIQVDDHMVTSDPDVLAVGEC
VEHDGNVYGLVAPLWDMCRSLADGLTDQHSGYKGSVTSTKLKVAGLDVFSAGDFSGGEGC
EDIVLRDASRGVYKRVIVKDDKLIGAVLYGDTVDGGWYFDLLKREENVADMRDMLIFGQA
FASGGGALDPKAAVAALSDEAEICGCNGVSKGQVVSCIAKGAHSLDAVRATCKASASCGS
CTGLVETLLALTLGEDVQSGPKTMCKCTSFTHDDVRREIVAQDMRSIPEVMQKLNWSTPE
GCSSCRPALNYYLLCALPGDYQDDQRSRFVNERMHANIQKDGTYSVVPRMWGGLTNPKEL
RAIADVVEKYNAPMVKVTGGQRLDIFGIRKEDLPAVWADLNAAGMVSGHAYGKSLRTVKT
CVGSEWCRFGTQDSTGLGVKLEHMTWGSWMPHKFKIAASGCPRNCAEATIKDFGVVCVDS
GYELHVGGNGGIHVRATDLLCKVATEQEAMDYCAAFIQLYREEARYLERTAPWIERVGVD
YVKSRIVADDVGREALRARFLFSQQFMQDDPWARRAAGEDAELHQHLAEVRPMETVA