Protein Info for PS417_06005 in Pseudomonas simiae WCS417

Annotation: lysyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 TIGR00499: lysine--tRNA ligase" amino acids 16 to 498 (483 residues), 668.1 bits, see alignment E=3.8e-205 PF01336: tRNA_anti-codon" amino acids 67 to 140 (74 residues), 38.6 bits, see alignment E=1.3e-13 PF00152: tRNA-synt_2" amino acids 157 to 495 (339 residues), 313.3 bits, see alignment E=2.4e-97

Best Hits

Swiss-Prot: 98% identical to SYK_PSEPF: Lysine--tRNA ligase (lysS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 98% identity to pfs:PFLU1228)

MetaCyc: 62% identical to lysine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Lysine--tRNA ligase. [EC: 6.1.1.6]

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U7Y1 at UniProt or InterPro

Protein Sequence (499 amino acids)

>PS417_06005 lysyl-tRNA synthetase (Pseudomonas simiae WCS417)
MSDQQLDQALQQEENSLIALRKEKLAAERAKGNAFPNDFRRENYCDALQKQYADKTKEEL
AEAAIPVKVAGRIMLNRGSFMVIQDMTGRIQVYVNRKTLPEETLASVKTWDMGDIIAAEG
TLARSGKGDLYVEMTHVRLLTKSLRPLPDKHHGLTDTEQRYRQRYVDLIVNEEVRHTFRV
RSQVIAHIRTFLMQRDFLEVETPMLQTIPGGAAAKPFETHHNALDMEMFLRIAPELYLKR
LVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYEDNMDLTEELFRELAQLVL
GSTDVPYGDKVFHFGEPFVRLSVFDSILKYNPELTADDLNDIDKARAIAKKAGAKVLGFE
GLGKLQVMIFEELVEHKLEQPHFITQYPFEVSPLARRNDDNPNVTDRFELFIGGREIANA
YSELNDAEDQAERFMAQVADKDAGDDEAMHYDADFVRALEYGMPPTAGEGIGIDRLVMLL
TNSPSIRDVILFPHMRPQA