Protein Info for GFF1173 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 363 (362 residues), 330.5 bits, E=1.2e-102
• PF13522: GATase_6
  • amino acids 34 to 160 (127 residues), 108.8 bits, E=3.1e-35
• PF13537: GATase_7
  • amino acids 48 to 165 (118 residues), 132 bits, E=1.9e-42
• PF00733: Asn_synthase
  • amino acids 211 to 357 (147 residues), 152.5 bits, E=3.8e-48
  • amino acids 357 to 503 (147 residues), 106 bits, E=5.3e-34

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (548 amino acids)

>GFF1173 Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MCSIFGIFNPAVSEQEARRIAVDQSNLMIHRGPDSHGVFSCPSGVLVHNRISIVDVFTGG
QPLYSQDQSVALVANGEIYNHHEVRQTFAQYPFATASDCEAILAAYMEEGVACLQRLRGM
FAFAILDQRTGTYLIGRDHMGILSLYYGHDASGALYVASEMKALVGVCERIEILPPGHYL
SSASPAPVQYYERDWQNFEGVRGWSSEVAALREALEESVESHMMSDVSFGLLLSGGLDSS
LVSSIAVRKYVQAGGRASDLCSFSIGLEGSPDLAAAREMAAFLGTRHFEHVYMIEEGFDA
IRKTIWHIESYDVTTVRASTPMQMLARKIRGNGVKVVLTGDGSDEAFGGYLYFHKAPGPR
ELHEECLRKLSMMHLYDCLRVSKTLLSWGVEPRVPFLDRQFLDVAMRIDPRFKMCGDGRI
EKHILRETFADYLPPSIAWRQKEQSSDGVGYEWIDGLKAMAAQAVTDRALQAAGHRFPHN
TPGSKEAYLYRSIFEELFPHRSAVFCAPGGKSAGNATEAASAWLGGQLIDDPSGRAVIGI
HASAMAAH