Protein Info for GFF117 in Sphingobium sp. HT1-2

Annotation: GTP-binding protein Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR00231: small GTP-binding protein domain" amino acids 5 to 159 (155 residues), 80.7 bits, see alignment E=1.1e-26 TIGR00436: GTP-binding protein Era" amino acids 6 to 276 (271 residues), 248.5 bits, see alignment E=7.7e-78 PF10662: PduV-EutP" amino acids 8 to 163 (156 residues), 22.2 bits, see alignment E=3.7e-08 PF02421: FeoB_N" amino acids 8 to 164 (157 residues), 51.9 bits, see alignment E=2.2e-17 PF01926: MMR_HSR1" amino acids 8 to 122 (115 residues), 82.7 bits, see alignment E=7.4e-27 PF07650: KH_2" amino acids 205 to 281 (77 residues), 60.6 bits, see alignment E=3.7e-20

Best Hits

Swiss-Prot: 64% identical to ERA_ERYLH: GTPase Era (era) from Erythrobacter litoralis (strain HTCC2594)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 93% identity to sch:Sphch_0772)

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>GFF117 GTP-binding protein Era (Sphingobium sp. HT1-2)
MNQRCGVIAIVGAPNAGKSTLVNALVGQKVAITSPKAQTTRTRVMGVAIEGDAQMVLVDT
PGIFAPRRRLDRAMVQAAWGGAQGADLIVLLVDGKAGLGAKMEPIIAALKARPERKWLVL
NKVDIAVKEKLLVHTQRLYEELEFEETFFVSAATGDGLPELKTAFANAMPEGPWHFPEDQ
VSDATDRMLAAEITREQLYHQLHAELPYAAAVDTEQYKERDDGSVEIHQQILVGRSSQRA
IVLGKGGQRLKEIGSKARAELANLLGVKVHLYLHVKVKEDWEEDRFIYRDIGLDWVD