Protein Info for GFF1158 in Variovorax sp. SCN45

Annotation: Acetolactate synthase large subunit (EC 2.2.1.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 26 to 588 (563 residues), 823.7 bits, E=3.5e-252
• PF02776: TPP_enzyme_N
  • amino acids 27 to 141 (115 residues), 142.4 bits, E=8.5e-46
• PF00205: TPP_enzyme_M
  • amino acids 216 to 352 (137 residues), 166.3 bits, E=4.9e-53
• PF02775: TPP_enzyme_C
  • amino acids 417 to 565 (149 residues), 178.7 bits, E=9.8e-57

Best Hits

Swiss-Prot: 59% identical to ILVI_ECOLI: Acetolactate synthase isozyme 3 large subunit (ilvI) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 99% identity to vpe:Varpa_2551)

MetaCyc: 76% identical to acetohydroxyacid synthase subunit B (Cupriavidus necator H16)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• superpathway of L-threonine metabolism (14/18 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• pyruvate fermentation to (R)-acetoin I (2/3 steps found)
• pyruvate fermentation to (S)-acetoin (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of (R,R)-butanediol biosynthesis (2/5 steps found)
• superpathway of 2,3-butanediol biosynthesis (2/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Protein Sequence (594 amino acids)

>GFF1158 Acetolactate synthase large subunit (EC 2.2.1.6) (Variovorax sp. SCN45)
MEISKAEIISAAAASSSAGNATQELMGAEVLVKALQAEGVQYVWGYPGGAVLYIYDAFYK
QDTIQHVLVRHEQAAVHAADGYARATGEVGVALVTSGPGLTNAVTGIATAYMDSIPMVII
SGQVPTAAIGLDAFQECDTVGITRPIVKHNFLVKDPKDLAMTMKKAFHIARSGRPGPVVV
DVPKDVSFKKVPYAGYPDKVEMRSYNPVRKGHGGQIRKALQLLLAAKRPYIYTGGGVLLG
NATNELRTLVDMLGYPVTNTLMGLGAYPASDRKFLGMLGMHGTIEANNAMQNCDVLLAVG
ARFDDRVIGNPKHFAQNERKIIHIDIDPSSISKRVKVDIPIVGDVKDVLTELISMIREST
AKPDAGALADWWKTIEAWRSRDCLKYDRGNKDVIKPQYVVETLWNMTKDADAYITSDVGQ
HQMWAAQYYRFDEPRRWINSGGLGTMGVGIPYAMGIKLAKPDSEVFTITGEGSVQMCIQE
LSTCLQYNTPIKICSLNNRYLGMVRQWQEIEYSGRYSHSYMDALPNFVKLAEAYGHVGML
IERPQDVEPALREARKLKDRTVFMDFRTDPTENVFPMVKAGMGITEMLLGSEDL