Protein Info for GFF1152 in Sphingobium sp. HT1-2

Annotation: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF01128: IspD" amino acids 11 to 228 (218 residues), 199.6 bits, see alignment E=9e-63 PF12804: NTP_transf_3" amino acids 13 to 137 (125 residues), 50.4 bits, see alignment E=4.6e-17 TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase" amino acids 13 to 227 (215 residues), 213.1 bits, see alignment E=3.4e-67 PF02542: YgbB" amino acids 235 to 387 (153 residues), 219.2 bits, see alignment E=5.2e-69 TIGR00151: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase" amino acids 235 to 387 (153 residues), 211.6 bits, see alignment E=6e-67

Best Hits

Swiss-Prot: 61% identical to ISPDF_SPHWW: Bifunctional enzyme IspD/IspF (ispDF) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K12506, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [EC: 2.7.7.60 4.6.1.12] (inferred from 71% identity to sch:Sphch_0701)

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis or Teichoic and lipoteichoic acids biosynthesis (EC 2.7.7.60, EC 4.6.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60 or 4.6.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (393 amino acids)

>GFF1152 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12) (Sphingobium sp. HT1-2)
MRRCMTSNSRIVALIVAAGQGSRAGGDIPKQYRRIGGKAVLAHAHDALTAHGGVSAIHIV
LGEGEEDRAQAILGNRVAHFVTGADSRRGSVRAGLEAIAAAGGADIVLIHDAARPFLPAC
VIDRLLAALEDAEGAVPTLPVADTLVRANGEIMADGVGRADLHRVQTPQAFRFETILAAH
RGWDEEQEATDDAQILRAWGHDVILVQGDERLEKLTYPQDFARAEARLGSARTTRVGMGY
DVHRLAPNEELWLGGVLVPHDRGLAGHSDADVALHAIVDAILGALAEGDIGSHFPPSDPQ
WRGASSDRFLAHARDLVLKRDGRIDHIDLTIICEAPKIGPHRDAMHVRIAEILGVPLDRV
SVKATTTERLGFAGRREGIAAQAVATLSLPTLF