Protein Info for GFF1146 in Variovorax sp. SCN45

Annotation: Pyrophosphate-energized proton pump (EC 3.6.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 838 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 57 to 75 (19 residues), see Phobius details amino acids 81 to 102 (22 residues), see Phobius details amino acids 123 to 149 (27 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 239 to 257 (19 residues), see Phobius details amino acids 263 to 283 (21 residues), see Phobius details amino acids 295 to 319 (25 residues), see Phobius details amino acids 331 to 351 (21 residues), see Phobius details amino acids 391 to 415 (25 residues), see Phobius details amino acids 471 to 488 (18 residues), see Phobius details amino acids 503 to 525 (23 residues), see Phobius details amino acids 575 to 594 (20 residues), see Phobius details amino acids 601 to 623 (23 residues), see Phobius details amino acids 666 to 685 (20 residues), see Phobius details TIGR01104: V-type H(+)-translocating pyrophosphatase" amino acids 8 to 684 (677 residues), 891 bits, see alignment E=3.4e-272 PF03030: H_PPase" amino acids 15 to 678 (664 residues), 920.9 bits, see alignment E=5.1e-281 PF00691: OmpA" amino acids 743 to 814 (72 residues), 43.6 bits, see alignment E=3.2e-15

Best Hits

Swiss-Prot: 79% identical to HPPA_RHOPL: K(+)-insensitive pyrophosphate-energized proton pump (hppA) from Rhodopseudomonas palustris

KEGG orthology group: K01507, inorganic pyrophosphatase [EC: 3.6.1.1] (inferred from 93% identity to vpe:Varpa_2539)

Predicted SEED Role

"Pyrophosphate-energized proton pump (EC 3.6.1.1)" in subsystem Phosphate metabolism (EC 3.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.1

Use Curated BLAST to search for 3.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (838 amino acids)

>GFF1146 Pyrophosphate-energized proton pump (EC 3.6.1.1) (Variovorax sp. SCN45)
MIGNTPLILAIVCGLVAVAYGFWARSWILARDPGNARMQEIAAAIQTGASAYLAKQYTTI
AVVGVVLAVLIALFLDLTTAVGFIVGAVLSGACGFIGMNVSVRANVRTAQAATHGIGPAL
DVAFRGGAITGMLVVGLGLLGVSGFYWFLAGSTPAAGQSLSAILNPLIGFAFGSSLISIF
ARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDCAGMAADLFETYA
VTLIATMVLGALMVTAAPTNAVLYPLALGGVSIIASIIGCFFVKASPGMTNVMPALYKGL
AVAGILSLIAFWFVTSWLIPDNAIAPSGSQLKLFGACFVGLALTAALVWITEYYTGTQYK
PVQHIAQASTTGHGTNIIAGLGVSMRSTAWPVIFVCIAILASYSLAGLFGIAVAATSMLS
MAGIVVALDAYGPITDNAGGIAEMSELPESVRAVTDPLDAVGNTTKAVTKGYAIGSAGLA
SLVLFADYTHKLESFGQAISFNLSDPMVIVGLFIGGLIPYLFGAMAMEAVGRAAGAVVEE
VRRQFRDIPGIMEGTGKPEYGKAVGMLTGAAIKEMMIPSLLPVVVPIVVGLLLGPKALGG
LLMGTIVTGLFVAISMCTGGGAWDNAKKYIEDGHHGGKGSEAHKAAVTGDTVGDPYKDTA
GPAVNPLIKIINIVALLIVPLVVKFHAGDAAAALPTKIETPPAATPPAAMAAPSTPGAVV
AAGAVSAAETAAVKVENGVVKFYFATASAEVAPNAREALADVIKAVQSGSTVLVSGYHDA
SGDPAKNAELAKQRAMAVSDALKAAGAPEDKIELAKPEQSQADGPPSEARRVEVKVKS