Protein Info for GFF1144 in Sphingobium sp. HT1-2

Annotation: Ribosome LSU-associated GTP-binding protein HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 TIGR03156: GTP-binding protein HflX" amino acids 17 to 385 (369 residues), 444.8 bits, see alignment E=9.9e-138 PF13167: GTP-bdg_N" amino acids 35 to 121 (87 residues), 88.8 bits, see alignment E=5.6e-29 PF16360: GTP-bdg_M" amino acids 124 to 203 (80 residues), 102 bits, see alignment E=4.2e-33 PF01926: MMR_HSR1" amino acids 211 to 313 (103 residues), 65.3 bits, see alignment E=1.1e-21 PF19275: HflX_C" amino acids 358 to 433 (76 residues), 29.4 bits, see alignment E=1.3e-10

Best Hits

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 88% identity to sch:Sphch_0708)

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (443 amino acids)

>GFF1144 Ribosome LSU-associated GTP-binding protein HflX (Sphingobium sp. HT1-2)
MAIFNRDSEDEVARGARAVVVHAETHGGDRRDSDARLEEARGLALAIGIDVRAAQSFRVR
DRKPATLFGSGQVDQIATLVNQEEAELVIVDNALTPVQQSNLEKGTGAKVIDRTGLILEI
FGERAATNEGRLQVELAHLDYQAGRLVRSWTHLERQRGGFGFLGGPGETQIEADRRMIRD
RMAKIRRELDQVTKTRGLHRARRQRAPWPVIALVGYTNAGKSTLFNRLTGAEVMAEDLLF
ATLDPTMRQIVLPGLDKAILSDTVGFVSDLPTQLIAAFRATLEEVLSADIIVHVRDIAHP
DTEAQRDDVLDVLSELGVAGEGALERGDGEGDPPPIIEAWNKLDLLDAESAAQAREIAAR
HDDVVIISALTGEGVEGLQRAISSRLTRGARVHSLRLPIADGAALAWLHEHGEVLATRPS
EAEMQVDVRLSDSGLARFLKRDR