Protein Info for GFF1141 in Variovorax sp. SCN45

Annotation: Membrane-fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 328 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF16576: HlyD_D23" amino acids 42 to 285 (244 residues), 44.9 bits, see alignment E=1.7e-15 PF13533: Biotin_lipoyl_2" amino acids 49 to 90 (42 residues), 31.7 bits, see alignment 2.1e-11 PF13437: HlyD_3" amino acids 208 to 282 (75 residues), 26.7 bits, see alignment E=1.6e-09

Best Hits

Swiss-Prot: 42% identical to AN36_HELPJ: 36 kDa antigen (jhp_1381) from Helicobacter pylori (strain J99 / ATCC 700824)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 72% identity to del:DelCs14_2240)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (328 amino acids)

>GFF1141 Membrane-fusion protein (Variovorax sp. SCN45)
MMSAKTRKLIAILVALLVLALLVWGFWRASQPAPEVFQGQMEARETDVAGKVTARVAEVA
VKEGDRIQAGAVLVRMDSPEVRAKLAQATAAEQAAQAVAEKAQNGARPQEVEMARMQWQR
AETAAQLAQTSFRRVDGLAREGLVADQKRDEAEANWKASRDAAIAAKAQYDMARTGARPE
DKAAASAQARQVAGVVAEAKAAKAETELRSPVGGEVAKVLAKVGELSPQGVAVVTVVDLN
DQWVVLNVREDRLSRFALGNEFDARLPALPEGKRTARFKVYYSAVLPDFATWRATRGGAG
FDVRTFEVRARPLKPIDGARPGMSVLVD