Protein Info for GFF1132 in Xanthobacter sp. DMC5

Annotation: Phosphate import ATP-binding protein PstB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 283 TIGR00972: phosphate ABC transporter, ATP-binding protein" amino acids 37 to 283 (247 residues), 367 bits, see alignment E=2e-114 PF00005: ABC_tran" amino acids 52 to 207 (156 residues), 103.7 bits, see alignment E=1.2e-33

Best Hits

Swiss-Prot: 68% identical to PSTB_NITWN: Phosphate import ATP-binding protein PstB (pstB) from Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)

KEGG orthology group: K02036, phosphate transport system ATP-binding protein [EC: 3.6.3.27] (inferred from 73% identity to xau:Xaut_3537)

MetaCyc: 68% identical to phosphate ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.27

Use Curated BLAST to search for 3.6.3.27 or 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (283 amino acids)

>GFF1132 Phosphate import ATP-binding protein PstB (Xanthobacter sp. DMC5)
MSIHTPSSPPADGAAHAGLPAPMLFASRQATLPPVKLSVRHVSFYYGDAIALRDISVDLR
ANCVTAFIGPSGCGKSTLLRIFNRMFELYPDQRVEGDVLLDGMSILGDAMDPARVRARIG
MVFQKPTPFPMSIFDNVAIGLRINEELKPADLDLRVEEALRKAALWDEVKDILPQSGLSL
SGGQQQRLCIARTIALKPEVILLDEPCSALDPITTARIEETIEELKAHYTFVIVTHNLQQ
AARISDFTGFMYLGEMVEFAPTQDLFTHPRDKRTQDYVTGRFG