Protein Info for GFF112 in Xanthobacter sp. DMC5

Annotation: Penicillin-insensitive murein endopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF03411: Peptidase_M74" amino acids 48 to 291 (244 residues), 352.4 bits, see alignment E=6.5e-110

Best Hits

Swiss-Prot: 45% identical to MEPA_ESCF3: Penicillin-insensitive murein endopeptidase (mepA) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: K07261, penicillin-insensitive murein endopeptidase [EC: 3.4.24.-] (inferred from 87% identity to xau:Xaut_3098)

Predicted SEED Role

"Murein endopeptidase"

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>GFF112 Penicillin-insensitive murein endopeptidase (Xanthobacter sp. DMC5)
MRRLAAAAVIALLGTAAQAQPAAKPATPVPAKELFGRATQPAPMAARSIGFYSKGCLAGG
EALPVNGPNWQVMRLSRNRNWGHPDLVAFLERFSAQVPKVSGWQGILVGDMAQPRGGPML
TGHASHQIGLDADIWLTPSPGRELTREEREKLSASMMVRADRKDIDPANWRPDTWKVIRA
AAVEPRVERIFVNAAIKKAICREATGDRSFLAKVRPYWGHDYHMHIRLTCPASSPDCRPQ
EAPPTGDGCGSELDWWFTDAVLHPKPEKEPSKPKPPLTLADLPDACAAVLDAR