Protein Info for PS417_05625 in Pseudomonas simiae WCS417

Annotation: ribonuclease T

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 TIGR01298: ribonuclease T" amino acids 21 to 219 (199 residues), 372.6 bits, see alignment E=2.3e-116 PF00929: RNase_T" amino acids 31 to 205 (175 residues), 87.5 bits, see alignment E=7.4e-29

Best Hits

Swiss-Prot: 92% identical to RNT_PSEF5: Ribonuclease T (rnt) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03683, ribonuclease T [EC: 3.1.13.-] (inferred from 100% identity to pfs:PFLU1153)

MetaCyc: 64% identical to ribonuclease T (Escherichia coli K-12 substr. MG1655)
Ribonuclease D. [EC: 3.1.13.5]; 3.1.13.5 [EC: 3.1.13.5]; 3.1.13.- [EC: 3.1.13.5]

Predicted SEED Role

"Ribonuclease T (EC 3.1.13.-)" in subsystem tRNA processing (EC 3.1.13.-)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.5

Use Curated BLAST to search for 3.1.13.- or 3.1.13.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UGT1 at UniProt or InterPro

Protein Sequence (223 amino acids)

>PS417_05625 ribonuclease T (Pseudomonas simiae WCS417)
MSEDHYDDEHEHSGGGSRHPMAERFRGYLPVVIDVETGGFNCATDALLEIAATTIGMDEQ
GFVFPEHTHFFRVEPFEGANIEAAALEFTGIKLDHPLRMAVSEEAALTDIFRGVRKALKA
NGCKRAILVGHNSSFDLGFLNAAVARLDMKRNPFHPFSSFDTATLAGLAYGQTVLAKACQ
AAGIDFDGREAHSARYDTEKTAELFCGIVNRWKDMGGWEDFND