Protein Info for GFF1099 in Variovorax sp. SCN45

Annotation: Na+-driven multidrug efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 16 to 37 (22 residues), see Phobius details amino acids 44 to 72 (29 residues), see Phobius details amino acids 92 to 114 (23 residues), see Phobius details amino acids 128 to 146 (19 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 186 to 211 (26 residues), see Phobius details amino acids 240 to 263 (24 residues), see Phobius details amino acids 275 to 301 (27 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 348 to 366 (19 residues), see Phobius details amino acids 387 to 408 (22 residues), see Phobius details amino acids 420 to 441 (22 residues), see Phobius details PF01554: MatE" amino acids 19 to 175 (157 residues), 63.4 bits, see alignment E=1.1e-21 amino acids 245 to 403 (159 residues), 78.6 bits, see alignment E=2.2e-26 TIGR00797: MATE efflux family protein" amino acids 19 to 411 (393 residues), 226 bits, see alignment E=3.9e-71

Best Hits

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 85% identity to vpe:Varpa_2496)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (450 amino acids)

>GFF1099 Na+-driven multidrug efflux pump (Variovorax sp. SCN45)
VSAPSGERGLIARHAGTVLVGQLAVMAFGVTDTIIAGRYSEGALAALSVGAAIFVSVYVS
LMGVLQALLPVWAELHGAGRSNEVGRSVRQSLYLAAIAIVVGMAALLAPAAVLRLTQVPP
EMRGDVEMYLRVLAFALAPALLFRLFSTLNQSLGKPQLVTWLQLGSLFVKLPLSIWFTFG
GAGLPAMGLVGCGWATLCVNWAMLACAIWLLRSSPFYRGYRLWERLEKPDWHQIRLFAKL
GVPGGLAVLVEVTSFTLMALFIARLGTAAAAAHQIAANLMAVAYMVPLSLSIATSARVSF
WLGAGDAMRARHACRLGFEQTVLCALLFSTALVTFRHPLANVYSDNPAVVALAASLMLAV
AAYHLADAMQTLCVFLLRCYRVTLAPLVIYCTMLWGLGLGGSYLLAYHGLGPWTAMRSPL
AFWMMSAGALALTAMFFISLLRWTMKKRAA