Protein Info for GFF109 in Variovorax sp. SCN45

Annotation: Protein lysine acetyltransferase Pat (EC 2.3.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13607: Succ_CoA_lig" amino acids 147 to 283 (137 residues), 172.6 bits, see alignment E=1.1e-54 PF19045: Ligase_CoA_2" amino acids 296 to 434 (139 residues), 24.1 bits, see alignment E=9.6e-09 PF13549: ATP-grasp_5" amino acids 473 to 694 (222 residues), 283.1 bits, see alignment E=4e-88 PF13302: Acetyltransf_3" amino acids 738 to 883 (146 residues), 27.9 bits, see alignment E=1e-09 PF00583: Acetyltransf_1" amino acids 806 to 882 (77 residues), 42.3 bits, see alignment E=2.6e-14 PF13508: Acetyltransf_7" amino acids 808 to 883 (76 residues), 32.3 bits, see alignment E=3.2e-11

Best Hits

KEGG orthology group: K09181, hypothetical protein (inferred from 92% identity to vpe:Varpa_2988)

Predicted SEED Role

"Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (900 amino acids)

>GFF109 Protein lysine acetyltransferase Pat (EC 2.3.1.-) (Variovorax sp. SCN45)
MDKHFLNPLFAPASIVAFVGHADDPGGQTPAGRTLREAFRADRFDGTLRFLDVRTKGTLE
ELAQTHADLALIALAPRDVAAALEIAGRIGCTAAAVISSGIDADQAAQWRKIARREGVHL
LGPNTLGFQRPPLHLNASVAGPLASTGPLALVSQSGALTASMLDWARQNGVGFSSVVSLG
PHTSVDIPQVLDFLANDRATHSIIVYLEGIADARRFMSALRSASHAKPVVVLKAGRKPAG
NEAAQTHSAAIVGSDDVFDAALRRAGAVRVRSFVELFSAAKCLASRYRPVGKRLAVVTNG
GGPGVLAADWINEIGLDLGKLSAPVQKLMLPTLPPLASLADLIDLSEEAQPAHYRAAIDA
AWADSQVDGVLAIFSPKAGADAAATAKALADIPRPMNKPLLACWMGDSSVGKARAVLAEA
TIPSFRTPEAAVGAFGNIAAFYQNQQLLQQTPPPLSALAKPDIEGARLLIESVLAERRKV
LTEMESKSLLSSFHIPITRTLLARSANEAMMIATQLGFPVALKIDSPDIPHKSDVEGVAL
NVMNGTGARDTYVEMMERVARLAPEARINGVTVQKMVRARRGREIYVGLVTDQPFGPVIV
FGAGGTMIELLNDRAMELPPLNQFLARRLIERSRVAETLGEWRGASAVDMEALEHVLLRV
SEMVCELPELREMDINPIIVDESGAISVDARIVVDSSTQAVAGHLGNAGNGYQHLAIMPY
PARYRREWPLPGGGTYIVRPVHPNDAQMLQALVQGLSPESRWFRFVSRFHELPPSMLSRF
TLIDYDREMALVAVVMERSVSPDGTVLESERIVGVSRYITNPDQTSCEFSLVVADEFSGK
GIGSRLMESIIDVARDKGLSEIDGLVLANNPDMLKLVRRLGFTVKSFPEDPDFKLVTYML