Protein Info for GFF1089 in Variovorax sp. SCN45

Annotation: Polyphosphate kinase (EC 2.7.4.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 TIGR03705: polyphosphate kinase 1" amino acids 19 to 678 (660 residues), 865.3 bits, see alignment E=1.7e-264 PF13089: PP_kinase_N" amino acids 20 to 121 (102 residues), 99 bits, see alignment E=4.8e-32 PF02503: PP_kinase" amino acids 130 to 312 (183 residues), 193.8 bits, see alignment E=5.5e-61 PF17941: PP_kinase_C_1" amino acids 340 to 503 (164 residues), 226.7 bits, see alignment E=3.2e-71 PF13090: PP_kinase_C" amino acids 513 to 676 (164 residues), 182.7 bits, see alignment E=1e-57

Best Hits

Swiss-Prot: 49% identical to PPK1_NEIMB: Polyphosphate kinase (ppk) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 93% identity to vpe:Varpa_2486)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (712 amino acids)

>GFF1089 Polyphosphate kinase (EC 2.7.4.1) (Variovorax sp. SCN45)
MQSDPADPSLDVAAPTFTLLDRDHSILSFNERVLDWAHRPEVPLIERLRYLCIVSSNLDE
FFEVRAAPHLIAGSAGDHKGTYTVESFERLAEAAHTLVARQYALYNDELMPTFATHGIHI
ISHGERNPAQRKWVSEYFEREVRPLLIPVGLDPAHPFPQVANKSLNFIVRLGGKDAFGRE
NPIQIVKVPRVLPRLIKMPAKVSDGKTLFVALSSVVRAHLSSMFPGREVGDFSQFRVTRH
SDLAVDEEDVKNLRTALRQGLQHRHYGQAVRLEVSSSCAESLASFLLAQFNLPPQALYRV
HGPVNLARFTQLADLLDEPKLRFPPYPASYPVTLSPSQSFFDRLQRGDVLIHQPFESFDG
VLAFLREAVLDPQVLAIKQTIYRTGADSVLMDLLREAVRRGKEVTVVVELKARFDEEANI
NWAEMLESIGAQVVYGVVGLKTHAKMLLVTRREGKQMRRYGHLSTGNYNPRTAKLYTDIS
HLTADPLLTADMETVFVHLASQSRLPKLNRMWLAPFDLHKNLVTQIDALGVAAAAGQPTR
IVAKMNALTDEEIIAALIRAGQKGVKIDLIVRGACTLPAQVPGLTDNIRVRSVIGRFLEH
SRVFYFRNGEDESLYLSSADWMNRNMMRRIELAWPVTDPGLRQRLIDECLVAYLHDGRDA
WDLGGDGIYTRVNHDTHSGEAGASRMIEAHGAQAALMNRYASRGHLRDASSN