Protein Info for GFF1077 in Xanthobacter sp. DMC5

Annotation: L-threonine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00291: PALP
  • amino acids 36 to 319 (284 residues), 242.8 bits, E=5e-76
• TIGR01127: threonine ammonia-lyase
  • amino acids 40 to 413 (374 residues), 385.7 bits, E=1.1e-119
• PF01842: ACT
  • amino acids 347 to 408 (62 residues), 31.1 bits, E=1.6e-11

Best Hits

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 91% identity to xau:Xaut_4294)

Predicted SEED Role

"Threonine dehydratase (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-methionine degradation II (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• L-threonine degradation V (1/2 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.19

Protein Sequence (424 amino acids)

>GFF1077 L-threonine ammonia-lyase (Xanthobacter sp. DMC5)
MTVPATALEVEKDLQGDLPVTLADVEGAWPLVRASVLRTPTLRAPRLSAFTGAEVYVKYE
NLQATASFKERGALVKLSRLTPDEARRGAVAMSAGNHAQAVAYHGTRLGISTLIVMPETT
PQVKVAATEAFGAEVVLFGETVADAYEEAERISKAQGRVFVHPYDDVDVIAGQGSVALEM
IEDGPAFDAVLVPIGGGGLIAGMAVAFAGRSPATQVVGVETQLYPAMWTAMTGEARPCGG
PTLAEGIAVRNVGEITAQIVRRLVPHVVLVDETALERAVNAFLMHQKTLAEGAGAAGLAA
LIAEPERFRGKTVGLVVSGGNIDPRMIAGILLRELEREERIVSFRLTILDRPGMLGRIAT
LLGKLGANILEVHHRRTFLDVPAKGTRLDLTVETRDQAHAELIQKALEGEGLPVQRLGAG
GADW