Protein Info for GFF1070 in Sphingobium sp. HT1-2

Annotation: Glutamate--cysteine ligase (EC 6.3.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01436: glutamate--cysteine ligase" amino acids 14 to 457 (444 residues), 716.8 bits, see alignment E=3.8e-220 PF04107: GCS2" amino acids 35 to 454 (420 residues), 388.6 bits, see alignment E=1.8e-120

Best Hits

Swiss-Prot: 58% identical to GSH1_MEDTR: Glutamate--cysteine ligase, chloroplastic (GSH1) from Medicago truncatula

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 89% identity to sch:Sphch_2070)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>GFF1070 Glutamate--cysteine ligase (EC 6.3.2.2) (Sphingobium sp. HT1-2)
MSTRTDSGGHDPVIESRDQLIAAFAKGAKPKDRWRIGTEHEKFVYSTKDHHAPAYEEKGG
IHTLLIGLTRYGWSPVFEGENIIALSGADGTISLEPAGQLELSGAPLDNLHQTCAETGRH
LEQVKYVGDMLGLGFLGLGMWPDKTRAELPIMPKGRYDIMLRHMPRVGSMGLDMMLRTCT
IQTNLDYGSEADMVQKFRVSLALQPLGTALFANSPFTEGKPNGFLSYRSHIWSDTDPQRT
GMLPFVFEDGFGYERYADYALDVPMYFVYRDGKYIDAAGLSFRDFLDGKLSVLPGEKPTE
KDWEDHLSTAFPEVRLKSFLEMRGSDGGPWNRICALPALWVGLLYDQGALDAAWDLVKDW
SMEERQILRDSVPKLGLDAPVSGGRTLRDIAPQVLDIARSGLAARQRFNAAGDNETGYLS
ALDEVIATGKTPAERLLDRYHGEWGGDVSRVYAEESF