Protein Info for GFF1067 in Sphingobium sp. HT1-2

Annotation: putative phospholipase D family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 PF00614: PLDc" amino acids 133 to 157 (25 residues), 23.9 bits, see alignment (E = 3.3e-09) amino acids 346 to 371 (26 residues), 28.5 bits, see alignment (E = 1.2e-10) PF13091: PLDc_2" amino acids 258 to 384 (127 residues), 42.8 bits, see alignment E=4.6e-15

Best Hits

KEGG orthology group: None (inferred from 81% identity to sch:Sphch_2073)

Predicted SEED Role

"Phospholipase D/Transphosphatidylase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (488 amino acids)

>GFF1067 putative phospholipase D family protein (Sphingobium sp. HT1-2)
MSMTGLPTPGEDCWRIEQAVRASVIVDADAYFRHARAAMLKAKKRIMLIGWDFDAAISLI
REDQADDGAPVVIGDFISWLVERTPDLEIYLLRWDMGAMKSMVRPTNLFTTLKWMAHPRV
TVKLDGHHPPAASHHQKIVVIDDCFAFCGGIDMTGDRWDTRHHRDEDPARRHPDGSPYGP
WHDATTALQGPVAMALGDHARARWVGAGGDELAPVEGEYDCWPDALPVHFEHVDVAIARS
APKMDDQDELIEIKKLYLHQIAAARHCIYAESQYFASRRIAEAIAKRLGDPDGPDIVIIN
PLQADGWLEQQAMDTARARLFEALKERDPHGKLRIYHPFTTRGAPIYVHAKILIVDDRLI
RVGSSNMNNRSMRLDTECDVVIDTALPGNDGRQEAILAIRDDLIAEHLDLPRERVSAVIA
QRGLIAAIEELRTKPGRTLRLYEVPDLPEVQAWLADNEVLDPEGPDEMFEAVSERGLFRR
MKGWFGRS