Protein Info for GFF1063 in Xanthobacter sp. DMC5

Annotation: Bifunctional protein FolD protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 7 to 122 (116 residues), 141.1 bits, E=1.9e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 125 to 287 (163 residues), 223.2 bits, E=1.1e-70

Best Hits

Swiss-Prot: 90% identical to FOLD_AZOC5: Bifunctional protein FolD (folD) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 91% identity to xau:Xaut_4086)

MetaCyc: 61% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• folate transformations I (10/13 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
• L-histidine degradation III (1/6 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (291 amino acids)

>GFF1063 Bifunctional protein FolD protein (Xanthobacter sp. DMC5)
VIETKPIDGKAFAAGLRARIAEEVSALKASAGLTPGLAVVLVGEDPASQVYVRNKAAQTA
EAGMASFEHKLPADTPEADVLALVAKLNADPAVNGILVQLPLPAQIDATKVIAAIDPAKD
VDGFHVVNAGRLAVGLDALVPCTPLGCIMLLKHHLGSLAGLKAVVVGRSNIVGKPAAQLL
LKEDCTVTIAHSRTRDLPGECRTADILVAAVGRPEMVRGDWIKPGATVIDVGINRVPKED
GKTRLVGDVAYAEAQGIAGLITPVPGGVGPMTIACLLQNTLTAAKRQKGLA