Protein Info for GFF1059 in Xanthobacter sp. DMC5

Annotation: Phosphoribosylformylglycinamidine synthase subunit PurL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 PF18072: FGAR-AT_linker" amino acids 17 to 55 (39 residues), 44.8 bits, see alignment 2.1e-15 TIGR01736: phosphoribosylformylglycinamidine synthase II" amino acids 20 to 731 (712 residues), 951 bits, see alignment E=2.2e-290 PF00586: AIRS" amino acids 76 to 190 (115 residues), 96.1 bits, see alignment E=2.6e-31 amino acids 438 to 557 (120 residues), 89.3 bits, see alignment E=3.5e-29 PF02769: AIRS_C" amino acids 204 to 358 (155 residues), 129.1 bits, see alignment E=2.7e-41 amino acids 571 to 708 (138 residues), 72.5 bits, see alignment E=6.9e-24

Best Hits

Swiss-Prot: 86% identical to PURL_AZOC5: Phosphoribosylformylglycinamidine synthase subunit PurL (purL) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K01952, phosphoribosylformylglycinamidine synthase [EC: 6.3.5.3] (inferred from 92% identity to xau:Xaut_4090)

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.3

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (738 amino acids)

>GFF1059 Phosphoribosylformylglycinamidine synthase subunit PurL (Xanthobacter sp. DMC5)
VNLIPNTPRITPELVAEHGLKPEEYDHFVKLIGREPTITELGIVSAMWNEHCSYKSSKVW
LRTLPTTGKRVIQGPGENAGVVDIGDGLAVVFKMESHNHPSFIEPYQGAGTGVGGILRDV
FTMGARPIAALNALRFGDCHNLKTRRLLAGVVAGIGGYGNSFGVPTVGGSVGFHKRYNGN
ILVNAMAVGLAKTDEIFYAAATGVGRAIVYLGSKTGRDGIHGATMASAEFGADAEEKRPT
VQVGDPFAEKLLLEACLEIMQAGCVVAIQDMGAAGLTCSAVEMGAKGDLGVELNLDAVPC
RETGMTAYEMMLSESQERMLMVIADGKEDEAEAIFRKWGLDFAIVGHTTDTLRFVVKHKG
LVEADLPIKELGDEAPEYRRPYEEPAPRPEILPSDVRTTASVADALERLIASPDLCSKRW
VWEQYDHLILGNTVQRPGGDAAVVRVNDGPKALAMTTDVTPRYCEAGPFEGGKQAVAEAW
RNLTAVGATPIAVTDNLNFGNPEKPEIMGQFVACVKGIGAACEALDFPVVSGNVSLYNET
NGKAILPTPTIGGVGLIEDLDQTMTLDFKAAGEPVLLIGKTKGWLGASAFLATVCEREEG
APPPVDLIAEKRNGDFVRNLIRDGVATAVHDVSDGGLLVALAEMAMASGIGVSLEDAPDG
MDPYAWWFGEDQARYVVTVASGQGAEVIHKAEMAGVPVMKIGKTGGDRLIVPDDRSIVVE
SLKDRHETWLPIYMGASS