Protein Info for GFF1053 in Sphingobium sp. HT1-2
Annotation: Phosphoribosylamine--glycine ligase (EC 6.3.4.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 64% identical to PUR2_RHILO: Phosphoribosylamine--glycine ligase (purD) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 90% identity to sch:Sphch_2083)Predicted SEED Role
"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
- superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (425 amino acids)
>GFF1053 Phosphoribosylamine--glycine ligase (EC 6.3.4.13) (Sphingobium sp. HT1-2) MNILLLGGGGREHALAWKLAQSPRLSTLYAAPGNPGIAQHATLVDLDATDHRAVLDFCTR HSIALVVIGPEAPLVDGLADNLRTMGVAVFGPDKKPAQLEGSKGFTKDLCQRANIPTAGY IRVTSKDGAIAALDDFTLPVVIKADGLAAGKGVIIAETRQEALDALDTMFSGAFGKAGEE VVLEEFMTGEEASFFALTDGSAILPFGSAQDHKRVGDGDTGPNTGGMGAYSPARVLTPEL EAQVIERIIKPTVETLAAEGMPYSGVLYAGLMLTEEGPKLIEYNARFGDPECQVLMMRFD GDLVELLLAVAEGRLAEQGPVTLADRTALTIVMAANGYPGTPEKGGAIKGIDAAEAQGAR VFHAGTAEKDGMIVANGGRVLNVTATGDTVGAAQAAAYAAVDAIDFPTGFCRRDIGWREV AREAQ