Protein Info for GFF105 in Xanthobacter sp. DMC5

Annotation: Silver exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 849 transmembrane" amino acids 195 to 215 (21 residues), see Phobius details amino acids 227 to 245 (19 residues), see Phobius details amino acids 257 to 276 (20 residues), see Phobius details amino acids 296 to 315 (20 residues), see Phobius details amino acids 451 to 472 (22 residues), see Phobius details amino acids 478 to 501 (24 residues), see Phobius details amino acids 794 to 813 (20 residues), see Phobius details amino acids 819 to 843 (25 residues), see Phobius details PF04945: YHS" amino acids 40 to 86 (47 residues), 46.2 bits, see alignment 1.2e-15 amino acids 89 to 134 (46 residues), 39.4 bits, see alignment 1.7e-13 PF19335: HMBD" amino acids 150 to 176 (27 residues), 45.3 bits, see alignment (E = 2.3e-15) TIGR01511: copper-translocating P-type ATPase" amino acids 241 to 844 (604 residues), 594 bits, see alignment E=6.5e-182 TIGR01525: heavy metal translocating P-type ATPase" amino acids 259 to 843 (585 residues), 624.6 bits, see alignment E=3.9e-191 TIGR01494: HAD ATPase, P-type, family IC" amino acids 304 to 815 (512 residues), 293.9 bits, see alignment E=3.4e-91 PF00122: E1-E2_ATPase" amino acids 333 to 514 (182 residues), 194.7 bits, see alignment E=3.6e-61 PF00702: Hydrolase" amino acids 531 to 748 (218 residues), 112.2 bits, see alignment E=1.5e-35

Best Hits

Swiss-Prot: 62% identical to SILP_SALTM: Silver exporting P-type ATPase (silP) from Salmonella typhimurium

KEGG orthology group: None (inferred from 84% identity to xau:Xaut_3091)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (849 amino acids)

>GFF105 Silver exporting P-type ATPase (Xanthobacter sp. DMC5)
MAQDHSSHDHSSHGGDCCGGKVPPPLDLSAAARPALPETVKDPVCGMDVKTDPAAGKPTT
EHEGHTYFFCCNGCKTKFEAAPETYLAPVKDPVCGMMVSPGTAKYSARHEKKPYFFCSSS
CETKFKADPVRYVGGSAPPPPVEVPAGTIYTCPMDPEIRQIGPGICPICGMALEPEVVTL
DEGPNHELVDMTRRFWIGLALTLPVFVLEMGSHLFGWSPLPQQISNYVQFVLATPVVLRV
GWPFLQRGWLSLKTRHLNMFTLIAMGTGVAYLYSLVGTFAPDLFPHDFHAHGGGVPVYFE
AAAVITVLVALGQVLELRAREQTSSAIKALLSLAPKTALRIRADGSDETIDLGLVAPGDL
LRVRPGEKVPVDGTVADGRAVVDESLVTGESLPVSREPGEKVIGGTVNASGVFTMKAEKV
GRDTLLSQIVQMVAQAQRSRAPIQRLADQVSGWFVPLVFAAAVVAFAVWMAVGPEPRLTF
ALMAAVSVLIIACPCALGLATPMSVMVGVGRGAQLGVLVRNAEALERMEKVDVVVVDKTG
TLTVGKPKVIAVRAFNGRSEDEVLRLAAALERASEHPLGAAIVHEAAARELVTSEPSGVE
SPAGKGIVGQVEGHTIAVGHAAYMSEIGVHTDHQTAMAEGFRREGATAVFVAIDGKAAGL
VAIADPVKDTTEAALRRLQQHGVRVVMLTGDNRTTALAVAKRLGIIEVEAEVLPARKAEV
VERLRKEGHVVAMAGDGVNDAPALAVADVGIAMGTGTDIAMESAGITLLKGDLAGIAHAR
ALSRATMANIRRNLLFAFLYNAAGVPIAAGVLYPSLGLLLSPMVAAAAMALSSVSVIANA
LLLRTFTPR