Protein Info for PS417_05300 in Pseudomonas simiae WCS417

Annotation: glucose dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 41 to 59 (19 residues), see Phobius details amino acids 66 to 82 (17 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 16 to 804 (789 residues), 1160.2 bits, see alignment E=0 PF01011: PQQ" amino acids 232 to 259 (28 residues), 24.1 bits, see alignment (E = 3.4e-09) amino acids 310 to 341 (32 residues), 22.9 bits, see alignment (E = 8.4e-09) amino acids 464 to 489 (26 residues), 21.1 bits, see alignment (E = 3e-08) amino acids 735 to 769 (35 residues), 22.5 bits, see alignment (E = 1.1e-08) PF13360: PQQ_2" amino acids 241 to 405 (165 residues), 29.5 bits, see alignment E=8.6e-11 amino acids 685 to 773 (89 residues), 22.3 bits, see alignment E=1.4e-08

Best Hits

Swiss-Prot: 52% identical to DHGA_ACICA: Quinoprotein glucose dehydrogenase A (gdhA) from Acinetobacter calcoaceticus

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 97% identity to pfs:PFLU1086)

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TWH6 at UniProt or InterPro

Protein Sequence (805 amino acids)

>PS417_05300 glucose dehydrogenase (Pseudomonas simiae WCS417)
MSTDGASSPSRLLPRLLGVLLLIMGLALLAGGIKLTMLGGSLYYLLAGIGITLTGVLLLA
TRRAALGLYALVLFASTVWALWEVGLDWWQLVPRLALLFALGIVMLLPWFRRPLLRGQPA
PLGTGALSVAVVLAGATALASQFTNPGEMVKTGQLDRDAVPGMASAAPSQAEGDWNSYGR
SAYGDRYSPLAQITPENAHKLVPAWTYRTGDIPGPNDPGETTAENTPLKVNGMLYVCTPH
SQVIALDPDTGKEIWRFDPKISSMGAENFKGWAHMTCRGVSYHDDAAYASEQSPTGSASP
SAAPNACPKRIFVPTADTRLIALNADTGKMCEDFGDKGQVDLRANIGGFAPGGYYSTSPP
AVTKNLVVIGGHVTDNVSTDEPSGVIRAFDVHTGKLVWNWDSGNPDDTTPLAEGKTYTRN
SPNMWSMFAVDEKLGMLYLPMGNQMPDQYGGDRTDDSEKYAAGLTALDIDTGHVKWTFQF
THHDLWDMDVGGQPSLIDIKTEAGVKQAVMASTKQGSIYVLDRATGQPVVPIHEVAVPQG
AVAGDRTSPTQPKSDLNFMPPPLKERDMWGVTPFDQLLCRIDFKSMRYDGAFTPPSLQGS
IVYPGNFGVFDWGGISVDPVRQIAFVNPSYMAFKSKLIPAADIAKQGPRVSETEGVQPNK
GAPYGVILEAMLSPMGLPCQAPAWGYVAAVDLTTHKTIWMHKNGTVRDSAPVPIPLTMGV
PSLGGTFTTAGGVAFLSGTLDQYLRAYDVKNGKQLWEGRLPAGAQTTPMTYTGKDGKQYV
LVMAGGHGSLGTKQGDYVMAFKLPE