Protein Info for GFF1029 in Xanthobacter sp. DMC5

Annotation: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00097: hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
  • amino acids 48 to 304 (257 residues), 331.7 bits, E=1.3e-103
• PF08543: Phos_pyr_kin
  • amino acids 55 to 302 (248 residues), 328.9 bits, E=1.9e-102
• PF00294: PfkB
  • amino acids 143 to 280 (138 residues), 44.3 bits, E=1.5e-15

Best Hits

Swiss-Prot: 61% identical to THID_RHIME: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (thiD) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00941, hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [EC: 2.7.1.49 2.7.4.7] (inferred from 81% identity to xau:Xaut_4453)

MetaCyc: 57% identical to bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (Escherichia coli K-12 substr. MG1655)
Phosphomethylpyrimidine kinase. [EC: 2.7.4.7]; Hydroxymethylpyrimidine kinase. [EC: 2.7.4.7, 2.7.1.49]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7)" (EC 2.7.4.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• hydroxymethylpyrimidine salvage (2/2 steps found)
• thiamine diphosphate salvage II (4/5 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.49 or 2.7.4.7

Protein Sequence (310 amino acids)

>GFF1029 Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase (Xanthobacter sp. DMC5)
VLRWRRDRKALAGRAKVDKSAGTGKGGGSIIPAHRSLTSSMSRPIPIALTIAGSDSSGGA
GIQADLKTFSALGVYGASVITALTAQNTRGVSAIHDVPDDFIAAQMDAVFSDLTVKAVKI
GMLSRPGAIRTVAEGLARHKARPVVLDPVMVAASGARLLSDDSLNDLRTILIPAADLITP
NLPEAGALLGTEAATTEEEMQRQGAALLALGARAVLMKGGHGEGPESTDLLVTPDGTRRF
TAPRHDTRNTHGTGCTLSSAIAAGLASGLDLPEAVEKAKAYITGAIAAADRLDVGAGHGP
VHHFFDLWRD