Protein Info for GFF1026 in Sphingobium sp. HT1-2

Annotation: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (EC 1.1.1.127) @ 2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 TIGR01832: 2-deoxy-D-gluconate 3-dehydrogenase" amino acids 5 to 251 (247 residues), 401.4 bits, see alignment E=7.5e-125 PF00106: adh_short" amino acids 10 to 201 (192 residues), 200.8 bits, see alignment E=3.3e-63 PF08659: KR" amino acids 12 to 187 (176 residues), 47.7 bits, see alignment E=3.7e-16 PF01370: Epimerase" amino acids 13 to 129 (117 residues), 21.7 bits, see alignment E=2.4e-08 PF13561: adh_short_C2" amino acids 16 to 248 (233 residues), 212.2 bits, see alignment E=1.8e-66

Best Hits

Swiss-Prot: 59% identical to KDUD_ECOLI: 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (kduD) from Escherichia coli (strain K12)

KEGG orthology group: K00065, 2-deoxy-D-gluconate 3-dehydrogenase [EC: 1.1.1.125] (inferred from 82% identity to nar:Saro_3515)

MetaCyc: 59% identical to putative 2-keto-3-deoxy-D-gluconate dehydrogenase (Escherichia coli K-12 substr. MG1655)
2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase. [EC: 1.1.1.127]; 1.1.1.- [EC: 1.1.1.127]; RXN0-7101 [EC: 1.1.1.127]

Predicted SEED Role

"2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 1.1.1.125)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.125

Use Curated BLAST to search for 1.1.1.125 or 1.1.1.127

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (251 amino acids)

>GFF1026 2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase (EC 1.1.1.127) @ 2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125) (Sphingobium sp. HT1-2)
MSTIFDLGGKVAIVTGANTGIGQAIALSLAAAGADIAAVGRTPAEETVEKVRALGRKAEI
VSADLSSIEPVQRVVDETIEKLGGLDILINNAGIIRRADSVDFTEEDWDAVIDTNLKSTF
FLAQAAGRHMLAQGSGKIVNIASLLSFQGGIRVPSYTASKSGVAGLTKLLANEWAAKGVN
VNAIAPGYIATNNTAALQADETRNRQIQERIPAGRWGDPSDIGGAAVFLSSPAANYIHGH
VLAVDGGWLAR