Protein Info for PS417_05185 in Pseudomonas simiae WCS417

Annotation: signal peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 64 to 83 (20 residues), see Phobius details PF10502: Peptidase_S26" amino acids 60 to 263 (204 residues), 186.5 bits, see alignment E=1.9e-59 TIGR02227: signal peptidase I" amino acids 64 to 266 (203 residues), 166.4 bits, see alignment E=2.5e-53

Best Hits

Swiss-Prot: 97% identical to LEP_PSEFL: Signal peptidase I (lepB) from Pseudomonas fluorescens

KEGG orthology group: K03100, signal peptidase I [EC: 3.4.21.89] (inferred from 99% identity to pfs:PFLU1059)

Predicted SEED Role

"Signal peptidase I (EC 3.4.21.89)" in subsystem Signal peptidase (EC 3.4.21.89)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.89

Use Curated BLAST to search for 3.4.21.89

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UDC8 at UniProt or InterPro

Protein Sequence (284 amino acids)

>PS417_05185 signal peptidase (Pseudomonas simiae WCS417)
MSLNFPLLLVIAVAVCGLLALLDLVFFAPRRRAAIASYQGSVSQPDGVVIEKLNKEPLLV
EYGKSFFPVLFIVLVLRSFLVEPFQIPSGSMKPTLDVGDFILVNKFSYGIRLPVIDKKVI
EIGDPQRGDVMVFRYPSDPNVNYIKRVVGLPGDVIRYTSDKRLFINGESVAEKLVGSEPN
SLGSAELYQEKLGAVEHEIRKEMSRYRAQPDGQWKVPAGHYFMMGDNRDNSNDSRYWDDP
NIPKDLLGMVPDQNIVGKAFAVWMSWPEPKLSHLPNFSRVGLIK