Protein Info for GFF1022 in Sphingobium sp. HT1-2

Annotation: Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02754: CCG
  • amino acids 6 to 87 (82 residues), 53.1 bits, E=1.4e-18
  • amino acids 136 to 219 (84 residues), 52.5 bits, E=2.2e-18

Best Hits

Swiss-Prot: 48% identical to LUTA_BACVZ: Lactate utilization protein A (lutA) from Bacillus velezensis (strain DSM 23117 / BGSC 10A6 / FZB42)

KEGG orthology group: None (inferred from 65% identity to bid:Bind_0560)

MetaCyc: 46% identical to iron-sulfur oxidase subunit used in L-lactate utilization (Bacillus subtilis subtilis 168)
L-lactate dehydrogenase. [EC: 1.1.1.27]

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE" in subsystem Lactate utilization

MetaCyc Pathways

• heterolactic fermentation (16/18 steps found)
• homolactic fermentation (11/12 steps found)
• Bifidobacterium shunt (13/15 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (15/19 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate fermentation to (S)-lactate (1/1 steps found)
• pyruvate fermentation to acetate and lactate II (3/4 steps found)
• L-histidine degradation V (1/2 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.27

Use Curated BLAST to search for 1.1.1.27

Protein Sequence (252 amino acids)

>GFF1022 Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE (Sphingobium sp. HT1-2)
VTQHKVALFVTCLVDMMRPRIGFAAIRALEAAGCDVIVPEGQTCCGQPALNSGDTEHAIA
LAKQTIVALEPYEAVVVPSGSCGGTIRCHYPELFEHDPEWLPRAQAVAGKTWEIMAYLDE
QCGWKPEGVQVDAKATYHDSCSGLRELGVKAQPRRLLKPITGLELTPLAGEETCCGFGGT
FCVKYPAISNAIVGEKADAIEATGADLLLAGDLGCLMNMAGKLNRQGSKVRAFHAIELIA
GMGDGPAIGEEA