Protein Info for GFF1020 in Sphingobium sp. HT1-2

Annotation: rhamnogalacturonan acetylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13472: Lipase_GDSL_2" amino acids 34 to 202 (169 residues), 58 bits, see alignment E=1.9e-19 PF00657: Lipase_GDSL" amino acids 34 to 233 (200 residues), 40.5 bits, see alignment E=3.6e-14

Best Hits

Swiss-Prot: 40% identical to RHGT1_BACSU: Rhamnogalacturonan acetylesterase RhgT (rhgT) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 40% identity to gct:GC56T3_1523)

Predicted SEED Role

"rhamnogalacturonan acetylesterase" in subsystem L-rhamnose utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (268 amino acids)

>GFF1020 rhamnogalacturonan acetylesterase (Sphingobium sp. HT1-2)
MRIATKAAAALALLATPLPALATQPGDTILIASDSTAANYKKGNYPQTGWGMMLGCGLSP
EVRVSNHAMGGRSTRTFIEEGRWTKLMSELMPGDTVLIQFGHNDAYRAKPQRWAPAQTDY
RNNLLRFIWDVRGAGGTPVLVTPVTRRSFGADGKAKADFAEYSAVMRELAANAHVPIIDL
EALSRAWVDRAGPDKAKAYFLHYKAEDKMPGFPKGIDDDTHFSELGARNVADLVAGGLKA
LNLPISAKVLASRPALDRTTPLGRTECQ