Protein Info for GFF102 in Sphingobium sp. HT1-2

Annotation: Proline iminopeptidase (EC 3.4.11.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00561: Abhydrolase_1" amino acids 71 to 344 (274 residues), 56 bits, see alignment E=7.2e-19 PF12697: Abhydrolase_6" amino acids 73 to 349 (277 residues), 58.9 bits, see alignment E=1.9e-19 PF12146: Hydrolase_4" amino acids 99 to 187 (89 residues), 46.6 bits, see alignment E=4.1e-16

Best Hits

KEGG orthology group: None (inferred from 68% identity to sal:Sala_1200)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (363 amino acids)

>GFF102 Proline iminopeptidase (EC 3.4.11.5) (Sphingobium sp. HT1-2)
MRYSASACLALFFITSPVSAQQPADPYAPAKAIVADVNRIVTPNGVQENLVVTLGGARQA
INVRGADRANPILLYVHGGPGAVEMPFAWSFQRPWEDFFTVVQWDQRGAGKSYALNDPKA
IAPTMTLDRYRDDAIELIDYLKTRYGKRKVFLLGHSWGSAVGLSVAIKRPDLLYAYVGMG
QAIDFRAGERMGMDWTIAQARKAGNAEAVKAIEALAPYPDSGPFTIDKADGWRRYAIPYG
SLMADRPDPTLYFQTPRLSPDYTPEDRKAWGTGSEFSTTPLWPRVADVSFAKVHEMKLPV
IMLLGRTDYTVPSPLAAQWMDRLKAPAKKTIWFEHSAHMPMVEEPGLVLKALINDVRPLA
DQE