Protein Info for GFF1019 in Sphingobium sp. HT1-2

Annotation: L-rhamnose-1-dehydrogenase ( EC 1.1.1.173)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF00106: adh_short" amino acids 7 to 199 (193 residues), 178.3 bits, see alignment E=2e-56 PF08659: KR" amino acids 8 to 170 (163 residues), 57.1 bits, see alignment E=3.4e-19 PF13561: adh_short_C2" amino acids 13 to 249 (237 residues), 212.5 bits, see alignment E=1e-66

Best Hits

Swiss-Prot: 52% identical to RHAD_THEAC: L-rhamnose 1-dehydrogenase (NADP(+)) (rhaD) from Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)

KEGG orthology group: None (inferred from 80% identity to cse:Cseg_1768)

MetaCyc: 96% identical to NAD(P)+-dependent L-rhamnose 1-dehydrogenase (Sphingomonas sp. SKA58)
RXN-12158 [EC: 1.1.1.378]; L-rhamnose 1-dehydrogenase. [EC: 1.1.1.378, 1.1.1.173]

Predicted SEED Role

"L-rhamnose-1-dehydrogenase ( EC 1.1.1.173)" in subsystem L-rhamnose utilization (EC 1.1.1.173)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.173 or 1.1.1.378

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (254 amino acids)

>GFF1019 L-rhamnose-1-dehydrogenase ( EC 1.1.1.173) (Sphingobium sp. HT1-2)
MKLLEGKTVLVTGASTGIGRAAAIGAAQHGADVVINYAHSDGPAASCVAEIEALGQRAIA
VKGDVADPQTAQDFVAKAVEAFGKVDVMVSNAGICPFHGFLDMPVDVVERTFKVNLHGAY
FMVQAAAQQMVKQGHGGSIVAVSSISALVGGEFQTHYTPTKAGVHSLMQSTAIALGKYGI
RCNSVLPGTILTEINKDDLADVEKREYMEKRTPLGRLGAPEDLAGPIVFLASDMAAYVTG
AALLVDGGMYVNLQ