Protein Info for PS417_05090 in Pseudomonas simiae WCS417

Updated annotation (from data): ferric citrate outer membrane transporter (fecA)
Rationale: Specific phenotype on citrate; 63% identical to E. coli fecA; SEED_correct
Original annotation: transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 778 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF07660: STN" amino acids 58 to 108 (51 residues), 35.6 bits, see alignment 1.3e-12 TIGR01783: TonB-dependent siderophore receptor" amino acids 136 to 778 (643 residues), 399.4 bits, see alignment E=1.7e-123 PF07715: Plug" amino acids 136 to 248 (113 residues), 77.2 bits, see alignment E=2.7e-25 PF00593: TonB_dep_Rec_b-barrel" amino acids 328 to 745 (418 residues), 178.5 bits, see alignment E=8.8e-56

Best Hits

Swiss-Prot: 64% identical to FECA_ECOLI: Fe(3+) dicitrate transport protein FecA (fecA) from Escherichia coli (strain K12)

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 97% identity to pfs:PFLU1040)

MetaCyc: 64% identical to ferric citrate outer membrane transporter (Escherichia coli K-12 substr. MG1655)
RXN0-1684

Predicted SEED Role

"Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U572 at UniProt or InterPro

Protein Sequence (778 amino acids)

>PS417_05090 ferric citrate outer membrane transporter (fecA) (Pseudomonas simiae WCS417)
MPQQPTLLARTLRQLLLGASLSLTALPPVMAADAKPYHIAPTSLEAALNQFGREAGVLIS
FGSEVTAGMQSRGLSGNYGAADGLQKLLEGTGLQARAEGDNAYSLQPATAPASIELGTSS
VVGDWLGDAAQTHVFEHPGARDVIRREEFERQGATQAKDVLNRIPGVNAPDNNGTGSHDM
ALNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSFAPISMGNMDAVDVVRGGGAVRY
GPQNVGGVVNFVTRAIPDAPTVKGGLQTETSPSSSHDGFKTTGNLLAGGTADNGLGGAIL
YSGTRGGDWRENSNTRIDDLILKGKYQLDDANSFNAMAQYYEGQADMPGGLNVADYKADP
YQSTRPYDKFWGRRTMFNVGYRYEQDRREFTVNSFFTKTLRSGYLDQGTFLSLSPREYWV
RGIETRFAQGFDLGPTSHEVGVGYRYINEAGHELRYRTPIAANQQIPTTNSRNDRDTRGG
TEANAFFVDDRIDIGKWTITPGIRYEIIESQQTNNLTNVKYKGDYNTALPALNVLYHLTD
NWNLYANTEGSFGSVQYSQMPNRVTSGEVKPEKARTWELGTRYDDGTLRAEIGAFLINFD
NQYESNQTNDSVIARGETRHQGIETSINYALDGLSPALAGFDVYATYAYVDATIREDGPN
KGNRVPFSSKHKGTLGVGYTEGRWKLNLDSSYQSSQFADNANTEKETANGANGRIPGYML
FSSRAAYDFGPQLSDLNVAVGVKNIFNTQYFTRSFDDNNKGKYVGEPRTVYVQTSIAF