Protein Info for GFF1000 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 PF01420: Methylase_S" amino acids 5 to 169 (165 residues), 78.7 bits, see alignment E=3.2e-26 amino acids 208 to 393 (186 residues), 100 bits, see alignment E=9.2e-33

Best Hits

Swiss-Prot: 100% identical to T1S_SALTY: Type-1 restriction enzyme StySJI specificity protein (hsdS) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01154, type I restriction enzyme, S subunit [EC: 3.1.21.3] (inferred from 100% identity to seh:SeHA_C4930)

Predicted SEED Role

"Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (452 amino acids)

>GFF1000 Type I restriction-modification system, specificity subunit S (EC 3.1.21.3) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MCNLNPKLKLDDDLDVGFMPMAGVPTTYLGKCNFETKKWSEVKKGFTQFQNDDVIFAKIT
PCFENGKAVVIKEFPNGYGAGSTEYYVLRSINGLINPHWLFALVKTKDFLTNGALNMSGS
VGHKRVTKEFLENYGVPVPPLAEQKVIAEKLDTLLAQVDSTKARLEQIPQILKRFRQSVI
VAAVNGQLTKELHKKNKFKLTELNISIPSLWKISEIGQFADVKGGKRLPKGESLIAENTG
FPYIRAGQLKNGTVLPEGQLYLEEYIQKSISRYTVSSGDLYITIVGACIGDAGIIPDVYN
NANLTENAAKICNLNENIFNRFLSLWLRSSYLQDIINSEIKSGAQGKLALARIKSLPLIL
PPLQEQHEIVRRVEQLFAYADTIEKQVNNALTRVNSLTQSILAKAFRGELTAQWRAENPE
LISGENSAAALLEKIKAERAASGGKKTSRKKA