Protein Info for Echvi_4631 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: (p)ppGpp synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 740 PF13328: HD_4" amino acids 39 to 188 (150 residues), 148.7 bits, see alignment E=3.5e-47 TIGR00691: RelA/SpoT family protein" amino acids 39 to 738 (700 residues), 650.2 bits, see alignment E=2e-199 PF01966: HD" amino acids 58 to 157 (100 residues), 31.7 bits, see alignment E=5e-11 PF04607: RelA_SpoT" amino acids 248 to 362 (115 residues), 127.8 bits, see alignment E=7.1e-41 PF02824: TGS" amino acids 407 to 466 (60 residues), 76.3 bits, see alignment 4.4e-25 PF28438: RelA_RIS" amino acids 604 to 656 (53 residues), 54.5 bits, see alignment 2.3e-18 PF13291: ACT_4" amino acids 663 to 738 (76 residues), 66 bits, see alignment E=1.2e-21

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 70% identity to mtt:Ftrac_1136)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G7H1 at UniProt or InterPro

Protein Sequence (740 amino acids)

>Echvi_4631 (p)ppGpp synthetase, RelA/SpoT family (Echinicola vietnamensis KMM 6221, DSM 17526)
MINVDLEVERKEILKRYRKILRLAKPLLKDGDAKVIKKAFNVSSDAHKEMRRKSGEPYIY
HPLEVALVCVEEIGLGTTSIVAALLHDVVEDTEWELEDIEREFGPKVTKIIDGLTKISGV
FEYGSSQQAENFRKMLLTLSDDVRVILIKLADRLNNMRTLQSMPRHKQLKIASETMYLYA
PLAHRLGLYSIKSELEDLYLKYTDTETYQFVVGKIRETRLSRNKFIKSFTAPIERELTAQ
GFDFVIKGRPKSVYSIYNKMKKQNIPFEEVYDLFAIRVIIDSEDENEKADCWQVYSIVTD
FYRPNPDRLRDWISTPRANGYESLHTTVMSNTGQWVEVQIRTSRMDDIAERGYAAHWKYK
EKDLSPGKSGSGLDEWITQVRTLLESNDGSAIEFMDDFRGNLFQDEVFVFTPKGDLKVLP
FGATALDFAFEIHSEVGEKCIGAKINQRLVPINHKLKNGDQVEILTSKKQKPSEDWLKSV
ITSRAKAKIKDALKEEKKSLIMDGKEIVQRKLKQMKMDFSSVVVEQLRAFFETKTATEFY
FKVGKGIIDPTSIKSFKEFKQIKKKKTKSPMQHVTDESSFTKEIKNLKGPDHDQLLIGED
MDVVDYILAKCCNPIPGDDVFGFVTVNEGIKIHRTSCPNALELLSNHGNRVIKARWTSQQ
EIAFLAGLKIVGTDRVGLINDLTRVISSELKVNMRSITVDSDSGLFEGSIKLYVHSTNHL
DNLIHNLMEVQGVIKVTRFD