Protein Info for Echvi_4445 in Echinicola vietnamensis KMM 6221, DSM 17526

Annotation: Superfamily I DNA and RNA helicases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 PF00580: UvrD-helicase" amino acids 23 to 109 (87 residues), 53.5 bits, see alignment E=5.8e-18 amino acids 145 to 218 (74 residues), 34.6 bits, see alignment E=3.5e-12 PF13245: AAA_19" amino acids 27 to 218 (192 residues), 52.5 bits, see alignment E=1.2e-17 PF13361: UvrD_C" amino acids 351 to 557 (207 residues), 37.7 bits, see alignment E=3.5e-13

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 49% identity to gbe:GbCGDNIH1_1179)

Predicted SEED Role

"possible DNA helicase"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0G513 at UniProt or InterPro

Protein Sequence (581 amino acids)

>Echvi_4445 Superfamily I DNA and RNA helicases (Echinicola vietnamensis KMM 6221, DSM 17526)
MNSPAEKASEETLKKIYLALDNGNSFRVEAGAGAGKTYSLIKALHYLIEHKSAELQKGFQ
RIACITYTNVATEEIRNRIDNHPSVFVDTIHGFCWSILQGFQAQMRAQIELIGDEKLIEK
IEDAGGLRKQKIVYDLGYRSATEKEIFIHHDNVIQLFTALLSKEKFRSILKNQYPIIFID
EYQDTNEELGKAIISNLIESKSDMQIGLFGDHWQKIYENGIGGMVSVDIEEIGKKANFRS
EKNLVKFLNRMRPGLPQMEKDPSSAGEVKIFHSNNWIGVRRDGKGGGHWTGDLPEAEAGN
YFRAVKNKLIQEGWEFSFEKTKILMLTNSVLAREQGYSGITKVFRFSDDYLKMQNKYIDY
FVNILEPVSESFSKKKYGEMLKAIGIRTPRLKNHSDKKVWNDSLSKLIKNRNSGTIGDVI
DCLAETQKPRLSSKIEESEEKYSKLKDLTEFEDEADKKLVSKIKKLKSVKYSEVIELAKY
IDDKTPFSTNHGVKGAEFENVLVICGRGWNKYNWNQFLTWANDGVPDDKKESFERNRNLF
YVSCSRPKKRLAVLFTQELSSSAMATLNKWFLPENIFPIII